The elite and stochastic model for iPS cell generation: Multilineage-differentiating stress enduring (Muse) cells are readily reprogrammable into iPS cells
Version of Record online: 12 JUN 2012
Copyright © 2012 International Society for Advancement of Cytometry
Cytometry Part A
Special Issue: Cytometry in Stem Cell Research
Volume 83A, Issue 1, pages 18–26, January 2013
How to Cite
Wakao, S., Kitada, M. and Dezawa, M. (2013), The elite and stochastic model for iPS cell generation: Multilineage-differentiating stress enduring (Muse) cells are readily reprogrammable into iPS cells. Cytometry, 83A: 18–26. doi: 10.1002/cyto.a.22069
- Issue online: 19 DEC 2012
- Version of Record online: 12 JUN 2012
- Manuscript Accepted: 16 APR 2012
- Manuscript Revised: 2 APR 2012
- Manuscript Received: 24 FEB 2012
Additional Supporting Information may be found in the online version of this article.
|CYTO_22069_sm_SuppFig1.tif||14786K||Supplemental Figure 1 Determination of positive gates for CD105 and SSEA-3. Flow cytometry data of adult human dermal fibroblasts was obtained and analyzed as the dot plot of the fluorescent signal (Pacific Blue or FITC) vs SSC. After determination of the positive gates for CD105 and SSEA-3 in the dot plots obtained from the samples treated with secondary antibodies only (?-mouse IgG-Pacific Blue and ?-rat IgM-FITC, respectively), the samples treated with both primary and secondary antibodies are analyzed (CD105-Pacific Blue and SSEA-3-FITC, respectively). The rate of each marker positive cells was shown as Mean ± SEM.|
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