Isolation and differentiation properties of neural crest stem cells

Authors

  • Elisabeth Dupin,

    Corresponding author
    1. Department of Developmental Biology, Institut de la Vision, Research Center UMR INSERM S968/CNRS 7210, 17 rue Moreau, 75012 Paris, France
    • Department of Developmental Biology, Institut de la Vision, Research Center UMR INSERM S968/CNRS 7210, 17 rue Moreau, 75012 Paris, France.
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  • Juliana M. Coelho-Aguiar

    1. CNRS UPR3294 Laboratoire Neurobiologie et Développement, Institut de Neurobiologie Alfred Fessard, Avenue de la Terrasse, 91190 Gif-sur-Yvette, France
    2. Universidade Federal do Rio de Janeiro, Instituto de Ciencias Biomedicas, CCS-Bloco F, 21940-590 Rio de Janeiro, Brazil
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Abstract

A wide array of neural and non-neural cell types arises from the neural crest during vertebrate embryogenesis. The neural crest forms transiently in the dorsal neural primordium to yield migratory cells that will invade nearly all tissues and later, differentiate into bones and cartilages, vascular smooth muscle cells, connective tissues, neurons and glial cells of the peripheral nervous system, endocrine cells, and melanocytes. Due to the amazingly diversified array of cell types they generate, the neural crest cells represent an attractive model in the stem cell field. We review here in vivo and in vitro studies of individual cells, which led to the discovery and characterization of neural crest progenitors endowed with multipotency and stem cell properties. We also present an overview of the diverse types, marker expression, and locations of the neural crest-derived stem cells identified in the vertebrate body, with emphasis on those evidenced recently in mammalian adult tissues. © 2012 International Society for Advancement of Cytometry

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