Additional Supporting Information may be found in the online version of this article.

MIFlowCyt.doc523KSupporting Information: MIFlowCyt
CYTO_22204_sm_SuppFig1.eps341KSupplemental Fig. 1: Dose response curve of BTP1. Jurkat T cells transfected with a NFAT luciferase reporter plasmid were incubated with BTP1 or DMSO as control before the cells were stimulated with PMA/ionomycin for 5 h. The luciferase reporter gene assay was performed as described previously [Sieber et al. Eur J Immunol 2007;37:2617-2626]. Data were depicted as percent inhibition compared to the stimulated control sample without inhibitor. A four parameter curve fit was used to calculate the IC50 value. Data out of two independent experiments are shown as MW + SD.
CYTO_22204_sm_SupplTab.pdf127KSuppl. Tab. 1: Collection of all applied amounts of barcoding dyes and inhibitor concentrations

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