Kinetic analysis of intracellular Hoechst 33342—DNA interactions by flow cytometry: Misinterpretation of side population status?
Article first published online: 7 NOV 2012
Copyright © 2012 International Society for Advancement of Cytometry
Cytometry Part A
Special Issue: Cytometry in Stem Cell Research
Volume 83A, Issue 1, pages 161–169, January 2013
How to Cite
Smith, P. J., Wiltshire, M., Chappell, S. C., Cosentino, L., Burns, P. A., Pors, K. and Errington, R. J. (2013), Kinetic analysis of intracellular Hoechst 33342—DNA interactions by flow cytometry: Misinterpretation of side population status?. Cytometry, 83A: 161–169. doi: 10.1002/cyto.a.22224
- Issue published online: 19 DEC 2012
- Article first published online: 7 NOV 2012
- Manuscript Accepted: 6 OCT 2012
- Manuscript Revised: 14 SEP 2012
- Manuscript Received: 13 AUG 2012
- BBSRC (PJS and RJE). Grant Number: 75/E19292
- EPSRC Basic Technology Programme Grant (PJS and RJE). Grant Number: GR/S23483
- Cancer Research UK (PJS)
- An EPSRC CASE Award with BioStatus Ltd (LC & KP)
Additional Supporting Information may be found in the online version of this article.
|CYTO_22224_sm_SuppFig1.doc||375K||Supplementary Figure 1: ABCG2 protein immunofluorescence detection in A549 cells confirms heterogenous expression.|
|CYTO_22224_sm_SuppFig2.doc||615K||Supplementary data Figure 2: Flow cytometry contour plots: changes in red versus violet fluorescence and light scatter characteristics of permeabilised A549 cells (5x105 cells/mL) exposed to increasing concentrations of Hoechst 33342|
|CYTO_22224_sm_SuppInfo.doc||125K||Supporting Information: MIFlowCyt|
|CYTO_22224_sm_SuppTab1.doc||47K||Supplementary Information: Table 1: Step-by-step procedure for system set-up and kinetic analysis of Hoechst 33342 uptake|
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