Literature Cited

  • 1
    van der Merwe PA, Dushek O. Mechanisms for T cell receptor triggering. Nat Rev Immunol 2011;11:4755.
  • 2
    Li QJ, Dinner AR, Qi S, Irvine DJ, Huppa JB, Davis MM, Chakraborty AK.CD4 enhances T cell sensitivity to antigen by coordinating Lck accumulation at the immunological synapse. Nat Immunol 2004;5:791791.
  • 3
    Shaw AS, Chalupny J, Whitney JA, Hammond C, Amrein KE, Kavathas P, Sefton BM, Rose JK. Short related sequences in the cytoplasmic domains of CD4 and CD8 mediate binding to the amino-terminal domain of the p56lck tyrosine protein kinase. Mol Cell Biol 1990;10:18531853.
  • 4
    Turner JM, Brodsky MH, Irving BA, Levin SD, Perlmutter RM, Littman DR. Interaction of the unique N-terminal region of tyrosine kinase p56lck with cytoplasmic domains of CD4 and CD8 is mediated by cysteine motifs. Cell 1990;60:755755.
  • 5
    Filipp D, Leung BL, Zhang J, Veillette A, Julius M. Enrichment of lck in lipid rafts regulates colocalized fyn activation and the initiation of proximal signals through TCR alpha beta. J Immunol 2004;172:42664266.
  • 6
    Kim PW, Sun ZY, Blacklow SC, Wagner G, Eck MJ. A zinc clasp structure tethers Lck to T cell coreceptors CD4 and CD8. Science 2003;301:17251725.
  • 7
    Schwarzenbacher M, Kaltenbrunner M, Brameshuber M, Hesch C, Paster W, Weghuber J, Heise B, Sonnleitner A, Stockinger H, Schütz GJ. Micropatterning for quantitative analysis of protein-protein interactions in living cells. Nat Methods 2008;5:10531053.
  • 8
    Simons K, Ikonen E. Functional rafts in cell membranes. Nature 1997;387:569569.
  • 9
    Pike LJ.Rafts defined. J Lipid Res 2006;47:15971597.
  • 10
    Balamuth F, Brogdon JL, Bottomly K.CD4 raft association and signaling regulate molecular clustering at the immunological synapse site. J Immunol 2004;172:58875887.
  • 11
    Fragoso R, Ren D, Zhang X, Su MW, Burakoff SJ, Jin YJ. Lipid raft distribution of CD4 depends on its palmitoylation and association with Lck, and evidence for CD4-induced lipid raft aggregation as an additional mechanism to enhance CD3 signaling. J Immunol 2003;170:913913.
  • 12
    Hawash IY, Kesavan KP, Magee AI, Geahlen RL, Harrison ML. The Lck SH3 domain negatively regulates localization to lipid rafts through an interaction with c-Cbl. J Biol Chem 2002;277:56835683.
  • 13
    Weghuber J, Brameshuber M, Sunzenauer S, Lehner M, Paar C, Haselgrubler T, Schwarzenbacher M, Kaltenbrunner M, Hesch C, Paster W, et al. Detection of protein-protein interactions in the live cell plasma membrane by quantifying prey redistribution upon bait micropatterning. Methods Enzymol 2010;472:133133.
  • 14
    Weghuber J, Sunzenauer S, Brameshuber M, Plochberger B, Hesch C, Schutz GJ. in-vivo detection of protein-protein interactions on micro-patterned surfaces. J Vis Exp 2010:pii:1969.
  • 15
    Muhammad A, Schiller HB, Forster F, Eckerstorfer P, Geyeregger R, Leksa V, Zlabinger GJ, Sibilia M, Sonnleitner A, Paster W, et al. Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome. J Immunol 2009;182:76727672.
  • 16
    Zimmermann L, Paster W, Weghuber J, Eckerstorfer P, Stockinger H, Schütz GJ. Direct observation and quantitative analysis of Lck-exchange between plasma membrane and cytosol in living T cells. J Biol Chem 2010;285:60636063.
  • 17
    Hesse J, Sonnleitner M, Sonnleitner A, Freudenthaler G, Jacak J, Hoglinger O, Schindler H, Schutz GJ. Single-molecule reader for high-throughput bioanalysis. Anal Chem 2004;76:59605960.
  • 18
    Moertelmaier M, Brameshuber M, Linimeier M, Schütz GJ, Stockinger H. Thinning out clusters while conserving stoichiometry of labeling. Appl Phys Lett 2005;87:263903.
  • 19
    Madl J, Weghuber J, Fritsch R, Derler I, Fahrner M, Frischauf I, Lackner B, Romanin C, Schutz GJ. Resting state orai1 diffuses as homotetramer in the plasma membrane of live mammalian cells. J Biol Chem 2010;285:4113541135.
  • 20
    McAnaney TB, Zeng W, Doe CF, Bhanji N, Wakelin S, Pearson DS, Abbyad P, Shi X, Boxer SG, Bagshaw CR. Protonation, photobleaching, and photoactivation of yellow fluorescent protein (YFP 10C): A unifying mechanism. Biochemistry 2005;44:55105510.
  • 21
    Lin CH, Chen HY, Yu CJ, Lu PL, Hsieh CH, Hsieh BY, Chang YF, Chou C. Quantitative measurement of binding kinetics in sandwich assay using a fluorescence detection fiber-optic biosensor. Anal Biochem 2009;385:224224.
  • 22
    Burghardt TP, Thompson NL. Evanescent intensity of a focused Gaussian light-beam undergoing total internal-reflection in a prism. Opt Eng 1984;23:6262.
  • 23
    Gavutis M, Lata S, Piehler J. Probing 2-dimensional protein-protein interactions on model membranes. Nat Protoc 2006;1:20912091.
  • 24
    Waichman S, You C, Beutel O, Bhagawati M, Piehler J. Maleimide photolithography for single-molecule protein-protein interaction analysis in micropatterns. Anal Chem 2011;83:501501.
  • 25
    Jares-Erijman EA, Jovin TM. FRETimaging. Nat Biotechnol 2003;21:13871387.
  • 26
    Vogel SS, Thaler C, Koushik SV. Fanciful FRET. Sci STKE 2006;2006re2.
  • 27
    Kasai RS, Suzuki KGN, Prossnitz ER, Koyama-Honda I, Nakada C, Fujiwara TK, Kusumi A. Full characterization of GPCR monomer-dimer dynamic equilibrium by single molecule imaging. J Cell Biol 2011;192:463463.
  • 28
    Hern JA, Baig AH, Mashanov GI, Birdsall B, Corrie JE, Lazareno S, Molloy JE, Birdsall NJ. Formation and dissociation of M1 muscarinic receptor dimers seen by total internal reflection fluorescence imaging of single molecules. Proc Natl Acad Sci U S A 2010;107:26932693.
  • 29
    Huppa JB, Axmann M, Mortelmaier MA, Lillemeier BF, Newell EW, Brameshuber M, Klein LO, Schütz GJ, Davis MM. TCR-peptide-MHC interactions in situ show accelerated kinetics and increased affinity. Nature 2010;463:963963.
  • 30
    Low-Nam ST, Lidke KA, Cutler PJ, Roovers RC, van Bergen en Henegouwen, PM, Wilson, BS, Lidke, DS. ErbB1 dimerization is promoted by domain co-confinement and stabilized by ligand binding. Nat Struct Mol Biol 2011;18:12441244.
  • 31
    Eck MJ, Atwell SK, Shoelson SE, Harrison SC. Structure of the regulatory domains of the Src-family tyrosine kinase Lck. Nature 1994;368:764764.
  • 32
    Panchamoorthy G, Fukazawa T, Stolz L, Payne G, Reedquist K, Shoelson S, Songyang Z, Cantley L, Walsh C, Band H. Physical and functional interactions between SH2 and SH3 domains of the Src family protein tyrosine kinase p59fyn. Mol Cell Biol 1994;14:63726372.
  • 33
    Romir J, Lilie H, Egerer-Sieber C, Bauer F, Sticht H, Muller YA. Crystal structure analysis and solution studies of human Lck-SH3; zinc-induced homodimerization competes with the binding of proline-rich motifs. J Mol Biol 2007;365:14171417.
  • 34
    Lee-Fruman KK, Collins TL, Burakoff SJ. Role of the Lck Src homology 2 and 3 domains in protein tyrosine phosphorylation. J Biol Chem 1996;271:2500325003.
  • 35
    Weghuber J, Sunzenauer S, Plochberger B, Brameshuber M, Haselgrubler T, Schutz GJ. Temporal resolution of protein-protein interactions in the live-cell plasma membrane. Anal Bioanal Chem 2010;397:33393339.