Additional Supporting Information may be found in the online version of this article.

CYTO_22228_sm_SuppInfo.doc42KSupporting Information
CYTO_22228_sm_SuppFig1.tiff4027KSupporting Information Figure 1. DRAQ7 at different concentrations reporting cell death. Images of cells grown in medium with a low concentration of serum (2%), in the presence of 3, 5 or 10 μM of DRAQ7. HeLa cells expressing the linker histone H1 tagged with eGFP were fixed with 4% formaldehyde and permeabilised with ethanol prior to adding DRAQ7.All frames were collected using the scan speed of 100 Hz. The brightness and contrast were adjusted. ? function was changed only in the fluorescence images of DRAQ7 (γ = 0.5). Scale bars: 10 μm.
CYTO_22228_sm_SuppFig2.tiff4160KSupporting Information Figure 2. DNA content frequency histograms of the untreated (Ctrl) and treated with 3 μM DRAQ7 A549 cells as expressed by DAPI (top panels) or DRAQ7 (bottom panels) integrated fluorescence intensity measured by LSC. To demonstrate a decline in intensity of DAPI fluorescence of cells long-term exposed to DRAQ7 unlike in figure 4 the fluorescence intensities of DAPI were plotted without electronic compensation (scale-up).
MIFlowCyt-Item-Location.doc76KSupporting Information: MIFlowCyt

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