Ethics statement: All the treatments of animals (mice) in this research followed the guidelines of the Institutional Animal Care and Ethics Committee at Eotvos Lorand University that operated in accordance with permissions 22.1/828/003/2007 issued by the Central Agricultural Office, Hungary and all animal work was approved by the appropriate committee.
Membrane microdomain organization, calcium signal, and NFAT activation as an important axis in polarized Th cell function†
Version of Record online: 26 NOV 2012
Copyright © 2012 International Society for Advancement of Cytometry
Cytometry Part A
Volume 83A, Issue 2, pages 185–196, February 2013
How to Cite
Izsepi, E., Himer, L., Szilagyi, O., Hajdu, P., Panyi, G., Laszlo, G. and Matko, J. (2013), Membrane microdomain organization, calcium signal, and NFAT activation as an important axis in polarized Th cell function. Cytometry, 83A: 185–196. doi: 10.1002/cyto.a.22234
- Issue online: 28 JAN 2013
- Version of Record online: 26 NOV 2012
- Manuscript Accepted: 29 OCT 2012
- Manuscript Revised: 19 OCT 2012
- Manuscript Received: 28 MAR 2012
- Hungarian National Science Fund. Grant Numbers: OTKA 64696, OTKA CK 80935
- EU Social Fund. Grant Number: TÁMOP 4.2.1./B-09/1/KMR-2010-0003
Additional Supporting Information may be found in the online version of this article.
|CYTO_22234_sm_SuppFig1.eps||1258K||Supporting Information Figure 1. Gating strategy for analysis of Th hybridoma and spleen derived CD4+ T cells. Representative dot-plots of Th1 hybridomas are shown in panel A. Gate1 indicates the homogeneity in size, while Gate2 and 3 shows gating for CD4 and CD3 positivity, respectively. Panel B shows the gating strategy used during the CD4+ T cell enrichment from spleen cells. Gate1 indicates gating by size, Gate2 shows gating for double positive (CD3+,CD4+) cells in freshly isolated spleen cells. Gate3,4 and 5 represent gating for size, CD4 and CD3 positivity in Th1 cells differentiated in vitro from CD4+ spleen T cells. Gates for Th2 cells looked similarly (not shown).|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.