Literature Cited

  • 1
    Palmieri M,Nowell CJ,Condron M,Gardiner J,Holmes AB,Desai J,Burgess AW,Catimel B.Analysis of cellular phosphatidylinositol (3,4,5)-trisphosphate levels and distribution using confocal fluorescent microscopy.Anal Biochem2010;406: 4150.
  • 2
    Lindblad J,Wählby C,Bengtsson E,Zaltsman A.Image analysis for automatic segmentation of cytoplasms and classification of Rac1 activation.Cytometry A 2004;57A:2233.
  • 3
    Bengtsson E,Wahlby C.Robust cell image segmentation methods.Pattern Recognit Image Anal 2004;14:157167.
  • 4
    Yan P,Zhou X,Shah M,Wong STC.Automatic segmentation of high-throughput RNAi fluorescent cellular images.IEEE Trans Inf Technol Biomed 2008;12:109117.
  • 5
    Yu W,Lee HK,Hariharan S,Bu W,Ahmed S.Quantitative neurite outgrowth measurement based on image segmentation with topological dependence.Cytometry A 2009;75A:289297.
  • 6
    Yu W,Lee HK,Hariharan S,Bu W,Ahmed S.Evolving generalized Voronoi diagrams for accurate cellular image segmentation.Cytometry A 2010;77:379386.
  • 7
    Srinivasa G,Fickus MC,Guo Y,Linstedt AD,Kovacevic J.Active mask segmentation of fluorescence microscope images.IEEE Trans Image Process 2009;18:18171829.
  • 8
    Srinivasa G,Fickus MC,Gonzalez-Rivero MN,Hsieh SY,Guo Y,Linstedt AD,Kovacevic J.Active mask segmentation for the cell-volume computation and Golgi-body segmentation of hela cell images. In:Institute of Electrical and Electronic Engineers, editors.Biomedical Imaging: From Nano to Macro. 5th IEEE International Symposium. Paris, May 14–17, 2008.Piscataway, NJ:IEEE;2008. pp 348351.
  • 9
    Held C,Wenzel J,Webel R,Marschall M,Lang R,Palmisano R,Wittenberg T.Using multimodal information for the segmentation of fluorescent micrographs with application to virology and microbiology. In: Institute of Electrical and Electronic Engineers, editors.Proceedings of the 33rd Annual International Conference of the IEEE EMBS. Boston, Massachusetts, August 30 to September 3, 2011.Piscataway, NJ:IEEE;2011. pp 64876490.
  • 10
    Wittenberg T,Grobe M,Münzenmayer C,Kuziela H,Spinnler K.A semantic approach to segmentation of overlapping objects.Methods Inform Med 2004;43:343353.
  • 11
    Held C,Palmisano R,Häberle L,Hensel M,Wittenberg T.Comparison of parameter-adapted segmentation methods for fluorescence micrographs.Cytometry A 2011;79A:933945.
  • 12
    Boykov Y,Funka-Lea G.Graph cuts and efficient N-D image segmentation.Int J Comput Vis 2006;70:109131.
  • 13
    Boykov Y,Kolmogorov V.An experimental comparison of min-cut/max-flow algorithms for energy minimization in vision.IEEE Trans Pattern Anal Mach Intell 2004;26:11241137.
  • 14
    Boykov Y,Kolmogorov V.Computing geodesics and minimal surfaces via graph cuts. In: Institute of Electrical and Electronic Engineers, editors. Proceedings of the 9th IEEE International Conference on Computer Vision, ICCV '03. Washington, DC, October 13–16, 2003, Vol2.Piscataway, NJ:IEEE;2003. pp 2633.
  • 15
    Boykov Y,Veksler O,Zabih R.Fast approximate energy minimization via graph cuts.IEEE Trans Pattern Anal Mach Intell 2001;23:12221239.
  • 16
    Maška M,Daněk O,Ortiz-de-Solórzano C,Muñoz-Barrutia A,Kozubek M,García IF.A two-phase segmentation of cell nuclei using fast level set-like algorithms. In:Salberg AB,Hardeberg JY,Jenssen R, editors.Image Analysis. Proceedings of 16th Scandinavian Conference, SCIA 2009.Berlin:Springer;2009. pp 390399.
  • 17
    Buie HR,Campbell GM,Klinck RJ,MacNeil JA,Boyd SK.Automatic segmentation of cortical and trabecular compartments based on a dual threshold technique for in vivo micro-CT bone analysis.Bone 2007;41:505515.
  • 18
    Nilsson B,Heyden A.A fast algorithm for level set-like active contours.Pattern Recognit Lett 2003;24:13311337.
  • 19
    Goldberg DE.Genetic Algorithms in Search, Optimization, and Machine Learning.Reading, MA:Addison-Wesley;1989.
  • 20
    Wall M.GAlib: A C++ Library of Genetic Algorithm Components. Version 2.4.7. Available at:
  • 21
    Van Rijsbergen CJ.Information Retrieval,2nd ed.London:Butterworths;1979.
  • 22
    Carpenter AE,Jones TR,Lamprecht MR,Clarke C,Kang IH,Friman O,Guertin DA,Chang JH,Lindquist RA,Moffat J, et al.CellProfiler: Image analysis software for identifying and quantifying cell phenotypes.Genome Biol 2006;7:R100.
  • 23
    Osuna EG,Hua J,Bateman NW,Zhao T,Berget PB,Murphy RF.Large-scale automated analysis of location patterns in randomly-tagged 3T3 cells.Ann Biomed Eng 2007;35:10811087.
  • 24
    Ljosa V,Sokolnicki KL,Carpenter AE.Annotated high-throughput microscopy image sets for validation.Nat Methods 2012;9:637.
  • 25
    Plant AL,Elliott JT,Bhat TN.New concepts for building vocabulary for cell image ontologies.BMC Bioinformatics 2011;12:487.
  • 26
    Wenzel J,Palmisano R,David JP,Wittenberg T,Lang R.Measurement of TLR-induced macrophage spreading by automated image analysis: Differential role of Myd88 and MAPK in early and late responses.Front Physiol 2011;2:71.
  • 27
    Webel R,Milbradt J,Auerochs S,Schregel V,Held C,Nöbauer K,Razzazi-Fazeli E,Jardin C,Wittenberg T,Sticht H,Marschall M.Two isoforms of the protein kinase pUL97 of human cytomegalovirus are differentially regulated in their nuclear translocation.J Gen Virol 2011;92:638649.