A mathematical model of natural killer cell activity

Authors

  • Anna Scherbakova,

    1. Department of Hematology and Oncology, University of Tartu, Tartu 51014, Estonia
    2. Department of Immunotherapy, Competence Centre for Cancer Research, Tallinn 12618, Estonia
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  • Helen Lust,

    1. Department of Hematology and Oncology, University of Tartu, Tartu 51014, Estonia
    2. Department of Immunotherapy, Competence Centre for Cancer Research, Tallinn 12618, Estonia
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  • Hele Everaus,

    1. Department of Hematology and Oncology, University of Tartu, Tartu 51014, Estonia
    2. Department of Immunotherapy, Competence Centre for Cancer Research, Tallinn 12618, Estonia
    3. Hematology and Bone Marrow Transplantation Unit, Department of Hematology and Oncology, Tartu University Hospital, Tartu 50417, Estonia
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  • Alar Aints

    Corresponding author
    1. Department of Hematology and Oncology, University of Tartu, Tartu 51014, Estonia
    2. Department of Immunotherapy, Competence Centre for Cancer Research, Tallinn 12618, Estonia
    3. Cell Therapy Laboratory, Department of Hematology and Oncology, Tartu University Hospital, Tartu 50417, Estonia
    • Cell Therapy Laboratory, Tartu University Hospital, Raja 31, 50417 Tartu, Estonia
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Abstract

Natural killer (NK) cells are capable of lysing their target cells with the help of perforin. The application of these cells for immunotherapy requires the estimation of their potency for the purpose of validation and batch-to-batch comparison. Cytotoxicity measurements have been carried out at only a few effector target ratios, therefore, allowing only semiquantitative assessment at best. By using a novel approach of varying the effector target ratio continuously and careful analysis of the experimental data after the reactions, we have achieved a precision necessary for constructing a mathematical model of cytotoxic reaction. Curve-fitting to experimental data indicates that NK cell cytotoxicity follows the law of mass action and fits the model of a single ligand–receptor interaction. The method allows to use the value of half-maximal lysis to describe the potency of cytotoxic NK cells numerically. © 2013 International Society for Advancement of Cytometry

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