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Additional Supporting Information may be found in the online version of this article.

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CYTO_22284_sm_SuppFig1.eps639KSupporting Information Figure 1. Dose dependent activation of YFP-AR-CFP and the untagged AR. The transcriptional activity of YFP-AR-CFP on a transiently transfected ARE2 TATA driven luciferase reporter gene is about 30% of the untagged variant. However, the minimal dose of R1881 to induce transcription is the same for untagged and double tagged ARs (0.1 nM) indicating that the dose dependency of AR activity is not changed by the two tags on YFP-AR-CFP. Means ± 2*SEM of minimally 2 independent experiments are shown.

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