Additional Supporting Information may be found in the online version of this article.

cytoa22287-sup-0001-SuppFig1.jpg108KSupporting Information Figure 1. Representative image of a histogram and bead map showing the doublet discriminator signal, which is a measure of apparent particle size, and the scatter plot of beads classification. The histogram shows the size of the particles which are passing through the flow cell. The software used for analysis (from Bio-Rad) only uses gated single bead events. Doublets and triplets are excluded from analysis. Bead regions (1−100): Axes are CL1 and CL2, representing the two dyes that label the beads. Classification 1 (first classification dye at 657 nm, embedded in each bead) and Classification 2 (second classification dye at 730 nm embedded in each bead).

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