Development of a bead-based multiplex assay for the simultaneous detection of porcine inflammation markers using xMAP technology
Article first published online: 10 APR 2013
Copyright © 2013 International Society for Advancement of Cytometry
Cytometry Part A
Volume 83A, Issue 7, pages 636–647, July 2013
How to Cite
Bongoni, A. K., Lanz, J., Rieben, R. and Banz, Y. (2013), Development of a bead-based multiplex assay for the simultaneous detection of porcine inflammation markers using xMAP technology. Cytometry, 83A: 636–647. doi: 10.1002/cyto.a.22287
- Issue published online: 20 JUN 2013
- Article first published online: 10 APR 2013
- Manuscript Accepted: 28 FEB 2013
- Manuscript Revised: 19 JAN 2013
- Manuscript Received: 23 OCT 2012
- Swiss National Science Foundation. Grant Number: 32003B_135272
Additional Supporting Information may be found in the online version of this article.
|cytoa22287-sup-0001-SuppFig1.jpg||108K||Supporting Information Figure 1. Representative image of a histogram and bead map showing the doublet discriminator signal, which is a measure of apparent particle size, and the scatter plot of beads classification. The histogram shows the size of the particles which are passing through the flow cell. The software used for analysis (from Bio-Rad) only uses gated single bead events. Doublets and triplets are excluded from analysis. Bead regions (1−100): Axes are CL1 and CL2, representing the two dyes that label the beads. Classification 1 (first classification dye at 657 nm, embedded in each bead) and Classification 2 (second classification dye at 730 nm embedded in each bead).|
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