Identification of perivascular mesenchymal stromal/stem cells by flow cytometry

Authors

  • Mirko Corselli,

    1. UCLA—Orthopaedic Hospital Department of Orthopaedic Surgery, The Orthopaedic Hospital Research Center, Los Angeles, California
    2. Eli and Edythe Broad Stem Cell Research Center, University of California, Los Angeles, California
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  • Mihaela Crisan,

    1. Department of Cell Biology, Erasmus MC Stem Cell Institute, Rotterdam, The Netherlands
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  • Iain R. Murray,

    1. Centre for Cardiovascular Science and Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom
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  • Christopher C. West,

    1. Centre for Cardiovascular Science and Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom
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  • Jessica Scholes,

    1. Eli and Edythe Broad Stem Cell Research Center, Flow Cytometry Core, University of California, Los Angeles, California
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  • Felicia Codrea,

    1. Eli and Edythe Broad Stem Cell Research Center, Flow Cytometry Core, University of California, Los Angeles, California
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  • Nusrat Khan,

    1. Centre for Cardiovascular Science and Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom
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  • Bruno Péault

    Corresponding author
    1. Eli and Edythe Broad Stem Cell Research Center, University of California, Los Angeles, California
    2. Centre for Cardiovascular Science and Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom
    • UCLA—Orthopaedic Hospital Department of Orthopaedic Surgery, The Orthopaedic Hospital Research Center, Los Angeles, California
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Correspondence to: Bruno Péault, Department of Orthopaedic Surgery, UCLA, Orthopaedic Hospital, The Orthopaedic Hospital Research Center, Los Angeles, CA, USA. E-mail: bpeault@mednet.ucla.edu

Abstract

Mesenchymal stem/stromal cells (MSCs) are adult multipotent progenitors of great promise for cell therapy. MSCs can mediate tissue regeneration, immunomodulation, and hematopoiesis support. Despite the unique properties of MSCs and their broad range of potential clinical applications, the very nature of these cells has been uncertain. Furthermore, MSCs are heterogeneous and only defined subpopulations of these are endowed with the particular abilities to sustain hematopoietic stem cells, regulate immune responses, or differentiate into mesodermal cell lineages. It is becoming evident that current criteria used to define cultured polyclonal MSCs (expression of nonspecific markers and in vitro mesodermal differentiation) are not sufficient to fully understand and exploit the potential of these cells. Here, we describe how flow cytometry has been used to reveal a perivascular origin of MSCs. As a result, the prospective purification of MSCs and specialized subsets thereof is now possible, and the clinical use of purified autologous MSCs is now within reach. © 2013 International Society for Advancement of Cytometry

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