Flow cytometric features of angioimmunoblastic T-cell lymphoma
Article first published online: 29 MAR 2006
Copyright © 2006 International Society for Analytical Cytology
Cytometry Part B: Clinical Cytometry
Volume 70B, Issue 3, pages 142–148, May/June 2006
How to Cite
Chen, W., Kesler, M. V., Karandikar, N. J., McKenna, R. W. and Kroft, S. H. (2006), Flow cytometric features of angioimmunoblastic T-cell lymphoma. Cytometry, 70B: 142–148. doi: 10.1002/cyto.b.20107
- Issue published online: 13 APR 2006
- Article first published online: 29 MAR 2006
- Manuscript Accepted: 20 JAN 2006
- Manuscript Received: 16 JUN 2005
- angioimmunoblastic T-cell lymphoma;
- flow cytometry;
The immunophenotypic features of angioimmunoblastic T-cell lymphoma (AILT) have not been well described.
We retrospectively reviewed our institutional experience with the flow cytometric features of 16 cases of AILT.
Multiparameter flow cytometry was able to identify a distinct population of immunophenotypically aberrant T cells in 15 of 16 cases. In 13 lymph node specimens, the neoplastic cells ranged from 1.9 to 87% (median 23%) of cells. The ratio of reactive to neoplastic T cells ranged from 0.01 to 20 (median 1.5); reactive T cells outnumbered neoplastic in 9/13 (69%) cases. The neoplastic populations expressed CD2, CD4, CD5, and CD45RO in all cases, lacked expression of CD8 and CD56 in all cases, and showed negative or dim surface CD3 in most cases. CD10 was expressed by the neoplastic populations in 11 of 14 cases at diagnosis; in 3 of these 11 only a subpopulation of the neoplastic cells was CD10(+). CD10 tended to be absent on neoplastic cells in staging bone marrows. The neoplastic population in all but one of the 15 positive cases possessed multiple immunophenotypic abnormalities and these were generally retained during the follow-up analyses of several cases.
These results indicate the potential utility of flow cytometry in the diagnosis and follow-up of AILT. © 2006 International Society for Analytical Cytology