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Simplified cytometry for routine monitoring of infectious diseases†‡
Article first published online: 28 JAN 2008
DOI: 10.1002/cyto.b.20405
Copyright © 2008 Clinical Cytometry Society
Issue

Cytometry Part B: Clinical Cytometry
Supplement: The Global Health and Diagnostic (Flow) Cytometry-Breakthrough in HIV and Tuberculosis
Volume 74B, Issue S1, pages S6–S10, 2008
Additional Information
How to Cite
Janossy, G. and Shapiro, H. (2008), Simplified cytometry for routine monitoring of infectious diseases. Cytometry, 74B: S6–S10. doi: 10.1002/cyto.b.20405
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How to cite this article: Janossy G, Shapiro H. Overview: Simplified cytometry for routine monitoring of infectious diseases. Cytometry Part B 2008; 74B (Suppl. 1): S6–S10, 2008.
Publication History
- Issue published online: 19 MAR 2008
- Article first published online: 28 JAN 2008
- Manuscript Accepted: 17 DEC 2007
- Manuscript Received: 16 DEC 2007
LITERATURE CITED
- 1. CD4: 20 years and counting. Cytometry (Clin Cytometry) 2002; 50: 39–132.Direct Link:
- 2,,,. T-cell subset counting and the fight against AIDS: Reflections over a 20-year struggle. Cytometry (Clin Cytometry) 2002; 50: 39–45.Direct Link:
- 3,,,,,. The role of clinical flow cytometry in the interferon-γ based diagnosis of active tuberculosis and its co-infection with HIV-1—A technically oriented review. Cytometry Part B (Clin Cytometry) 2008; 74B ( Suppl). In press. doi: 10.1002/cyto.b.20381 (this issue).
- 4,. ‘Killer’ applications: Towards affordable rapid cell-based diagnostics for malaria and tuberculosis. Cytometry Part B (Clin Cytometry) 2008; 74B ( Suppl). In press. doi: 10.1002/cyto.b.20401 (this issue).
- 5,,,,,,. Pneumocystis carinii pneumonia and mucosal candidiasis in previously healthy homosexual men: Evidence of a new acquired cellular immunodeficiency. New Engl J Med 1981; 305: 1425–1431.
- 6
- 7,. Biomedical Platforms—Realigning the Normal and the Pathological in Late-Twentieth-Century Medicine. London: MIT; 2003.
- 8,,,,,,. The prognostic value of cellular and serologic markers in infection with human immunodeficiency virus type 1. N Engl J Med 1990; 322: 166–172.
- 9,,,,,,,,. Use of CD45 gating in three and four-color flow cytometric immunophenotyping: Guideline from the National Institute of Allergy and Infectious Diseases. Division of AIDS. Cytometry (Clin Cytometry) 2002; 50: 46–52.Direct Link:
- 10,,,,,,. Quality control of CD4+ T-lymphocyte enumeration: Results from the last 9 years of the UK National External Quality Assessment Scheme for Immune Monitoring. Cytometry (Clin Cytometry) 2002; 50: 102–110.Direct Link:
- 11,,,,. Impact of the international program for Quality Assessment and Standardization for Immunological Measures Relevant to HIV/AIDS: QASI. Cytometry (Clin Cytometry). 2002; 50: 111–116.Direct Link:
- 12,,. Seventeen-colour flow cytometry: Unravelling the immune system. Nat Rev Immunol 2004; 4: 648–655.
- 13,. Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling. Nat Methods 2006; 3: 361–368.
- 14. Why and how to quantify minimal residual disease in acute lymphoblastic leukemia? Leukemia 2007; 21: 622–626.
- 15,,,,. Quality assurance for polychromatic flow cytometry. Nat Protocol 2006; 1: 1522–1530.
- 16,,,,. Interpreting flow cytometry data: A guide for the perplexed. Nat Immunol 2006; 7: 681–685.
- 17,,. Guidelines for performing single-platform absolute CD4+ T-cell determinations with CD45 gating for persons infected with human immunodeficiency virus. Centers for Disease Control and Prevention. MMWR Recomm Rep 2003; 52: 1–13.
- 18,,,,,. Precise CD4 T-cell counting using red diode laser excitation: For richer, for poorer. Cytometry (Clin Cytometry) 2002; 50: 78–85.Direct Link:
- 19,,,,,,,,. Large-scale affordable panleucogated CD4+ testing with proactive internal- and external quality assessment—in support of the South African National Comprehensive Care, Treatment, and Management Programme for HIV/AIDS. Cytometry Part B (Clin Cytometry) 2008; 74B ( Suppl): in press. doi: 10.1002/cyto.b.20384 (this issue).
- 20,,,,,. Performance of the panleucogating protocol for CD4+ T cell enumeration in an HIV dedicated laboratory facility in Barbados. Cytometry Part B (Clin Cytometry) 2008; 74B ( Suppl). In press. doi: 10.1002/cyto.b.20406 (this issue).
- 21,,,,,,,,,. A multi-laboratory study of CD4 counts using Flow Cytometric PanLeukogating (PLG): A NIAID-DAIDS Immunology Quality Assessment Program study. Cytometry Part B (Clin Cytometry) 2008; 74B ( Suppl). In press doi: 10.1002/cyto.b.20417 (this issue).
- 22CLSI. Laboratory documents: Enumeration of immunologically defined cell populations by flow cytometry. Approved Guideline, 2nd ed. CLSI document H42-A2. Wayne, PA: CLSI; 2007.
- 23,,,,,,,,,,. Performance evaluation of the FACSCount System: A dedicated system for clinical cellular analysis. Cytometry 1996; 26: 52–59.Direct Link:
- 24,. The role of CD4 T cell measurements in the management of anti-retroviral therapy—A review with an emphasis on pediatric patients. Cytometry Part B (Clin Cytometry) 2008; 74B ( Suppl). In press. doi: 10.1002/cyto.b.20398 (this issue).
- 25,,,,,,. Validation of primary CD4 gating as an affordable strategy for absolute CD4 counting in Cambodia. J AIDS 2006; 43: 179–185.
- 26,,,,,,,,,. Evaluation of a low-cost method, the Guava EasyCD4 assay, to enumerate CD4-positive lymphocyte counts in HIV-infected patients in the United States and Uganda. J AIDS 2006; 41: 607–610.
- 27,,,,,,,,,,. Cost-effectiveness of HIV treatment in resource-poor settings—the case of Côte d'Ivoire. N Engl J Med 2006; 355: 1141–1153.
- 28,. Clinical applications and availability of CD4+ T cell count testing in sub-Saharan Africa. Cytometry Part B (Clin Cytometry) 2008; 74B ( Suppl). In press. doi: 10.1002/cyto.b. 20383 (this issue).
- 29World Health Organization. Emergency scale-up of antiretroviral therapy in resource-poor settings: Technical operational recommendations to achieve 3 by 5; 2004.http://www.who.int/3by5/publications/documents/en/zambiaen.pdf.
- 30,,,,. African regional external quality assessment (AFREQAS) for CD4 T-cell enumeration: Development, outcomes, and performance of laboratories. Cytometry Part B (Clin Cytometry) 2008; 74B ( Suppl). In press. doi: 10.1002/cyto.b.20397 (this issue).
- 31,,,,. Laboratory medicine in Africa: A barrier to effective health care. Clin Infect Dis 2006; 42: 377–382.
- 32,,. Properly functioning scientific equipment in developing countries. Anal Chem 78: 5273–5276.
- 33. The fragile environments of Inexpensive CD4+ T cell enumeration in the least developed countries (LDC): Strategies for accessible support. Cytometry Part B (Clin Cytometry) 2008; 74B ( Suppl). In press. doi: 10.1002/cyto.b.20386 (this issue).
- 34,,,,,,,. Comparative assessment of five alternative methods for CD4+ T-lymphocyte enumeration for implementation in developing countries. J AIDS 2001; 26: 193–195.
- 35. Health care reform and the crisis of HIV and AIDS in South Africa. N Engl J Med 2004; 351: 81–92.
- 36,,,,,. Rapid expansion of the health workforce in response to the HIV epidemic. N Engl J Med 2007; 357: 2510–2514.
- 37. Affordable diagnostics: Changing the paradigm in India. Cytometry Part B (Clin Cytometry) 2008; 74B ( Suppl). In press. doi: 10.1002/cyto.b.20402 (this issue).
- 38,. Flow cytometry as the spearhead for delivering sustainable and versatile laboratory services to HIV-burdened health care systems of the developing world: A Caribbean model with potentially universal application. Cytometry Part B (Clin Cytometry) 2008; 74B ( Suppl). In press. doi: 10.1002/cyto.b. 20400 (this issue).
- 39,,,. Multiplexed immunoassays by flow cytometry for diagnosis and surveillance of infectious diseases in resource-poor settings. Lancet Infect Dis 2002; 2: 243–250.
- 40. The changing pattern of “smart” flow cytometry (S-FC) to assist the cost-effective diagnosis of HIV, tuberculosis and leukemias in resource-restricted conditions. Biotechnol J 2008; 3: doi: 10.1002.biot 200700200.Direct Link:
- 41,,,,,,,. CD8/CD38 activation yields important clinical information of effective anti-retroviral therapy (ART): Findings from the first year of the CIPRA-SA cohort. Cytometry Part B (Clin Cytometry) 2008; 74B ( Suppl). In press. doi: 10.1002/cyto.b.20391 (this issue).
- 42,,,,,,,,,,,. Loss of receptor on tuberculin-reactive T-cells marks active pulmonary TB. PLoS ONE 2007; 2: e735.
- 43: “Cellular astronomy”—A foreseeable future in cytometry. Cytometry Part A 2004; 60A: 115–124.Direct Link:
- 44,. Personal cytometers—Slow flow or no flow? Cytometry Part A 2006; 69A: 620–630.Direct Link:
- 45,,,,,,,,,,,,,,. A microchip CD4 counting method for HIV monitoring in resource-poor settings. PLoS Med 2005; 2: e182.
- 46,,,,,,. A single platform image cytometer for resource-poor settings to monitor disease progression in HIV infection. Cytometry Part A 2007; 71A: 132–142.Direct Link:
- 47,,,,. An immunomagnetic single-platform image cytometer for cell enumeration basedon antibody specificity. Clin Vaccine Immunol 2007; 14: 412–419.
- 48,,,,,,. CD4(+) T lymphocytes enumeration by an easy-to-use single platform image cytometer for HIV monitoring in resource-constrained settings. Cytometry Part B (Clin Cytometry) 2007; 72B: 397–407.Direct Link:
