Altered T cell differentiation associated with loss of CD27 and CD28 in HIV infected Indian individuals

Authors

  • Kamalika Mojumdar,

    1. HIV & Immunology Laboratory, Department of Microbiology, All India Institute of Medical Sciences, New Delhi 110029, India
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  • Madhu Vajpayee,

    Corresponding author
    1. HIV & Immunology Laboratory, Department of Microbiology, All India Institute of Medical Sciences, New Delhi 110029, India
    • Laboratory Head, HIV & Immunology Division, In charge, HIV National Reference Laboratory & Integrated Counseling and Testing Center, Department of Microbiology, AIIMS, New Delhi 110029, India
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  • Neeraj Kumar Chauhan,

    1. HIV & Immunology Laboratory, Department of Microbiology, All India Institute of Medical Sciences, New Delhi 110029, India
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  • Alpana Singh,

    1. HIV & Immunology Laboratory, Department of Microbiology, All India Institute of Medical Sciences, New Delhi 110029, India
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  • Ravinder Singh,

    1. HIV & Immunology Laboratory, Department of Microbiology, All India Institute of Medical Sciences, New Delhi 110029, India
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  • Sravya Kurapati

    1. HIV & Immunology Laboratory, Department of Microbiology, All India Institute of Medical Sciences, New Delhi 110029, India
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  • How to cite this article: Mojumdar K, Vajpayee M, Chauhan NK, Singh A, Singh R, Kurapati S. Altered T cell differentiation associated with loss of CD27 and CD28 in HIV infected Indian individuals. Cytometry Part B 2012; 82B: 43–53

Abstract

Background:

HIV-1 infection is associated with depletion of naïve T cell subsets and skewed T cell differentiation and maturation, leading to accumulation of T cells at intermediate and end stages of differentiation. CD27 and CD28 expression have been utilized in assessing these population subsets.

Methods:

We characterized T cell subsets based on expression of CD45RA, CCR7, CD27, and CD28 and compared these subsets in HIV-1 infected Indian subjects and uninfected controls.

Results:

HIV-1 infection was associated with an increase in effector and memory T cell subsets and a concomitant decrease in naïve T cells. HIV-1 infected subjects showed accumulation of intermediate CD8 T cell (CD27+CD28−) differentiation subsets, whereas CD4 T cells progressed to late stage differentiation (CD27−CD28−). These subsets were negatively associated with CD4 T cell counts and positively associated with plasma viremia. CD57, an immunosenescence marker, was also increased on T cell subsets from HIV-1 infected individuals. Antiretroviral therapy resulted in partial restoration of differentiation status.

Conclusion:

Persistent HIV-1 replication and chronic immune activation, along with altered cytokine secretion profile, lead to impaired T cell differentiation and maturation. Detailed understanding of factors associated with differentiation defects in HIV-1 infected Indian individuals will strongly assist in Indian HIV-1 vaccine efforts and add to our knowledge of HIV-1 subtype C pathogenesis. © 2011 International Clinical Cytometry Society

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