How to cite this article: Cesano A, Rosen DB, O'Meara P, Putta S, Gayko U, Spellmeyer DC, Cripe LD, Sun Z, Uno H, Litzow MR, Tallman MS, Paietta E. Functional pathway analysis in acute myeloid leukemia using single cell network profiling assay: effect of specimen source (bone marrow or peripheral blood) on assay readouts. Cytometry Part B 2012; 82B: 158–172.
Version of Record online: 14 FEB 2012
Copyright © 2012 International Clinical Cytometry Society
Cytometry Part B: Clinical Cytometry
Volume 82B, Issue 3, pages 158–172, May 2012
How to Cite
Cesano, A., Rosen, D. B., O'Meara, P., Putta, S., Gayko, U., Spellmeyer, D. C., Cripe, L. D., Sun, Z., Uno, H., Litzow, M. R., Tallman, M. S. and Paietta, E. (2012), Functional pathway analysis in acute myeloid leukemia using single cell network profiling assay: Effect of specimen source (bone marrow or peripheral blood) on assay readouts. Cytometry, 82B: 158–172. doi: 10.1002/cyto.b.21007
Conflict of Interest: A. C., D.B.R., P.O., S.P., U.G., and D.C.S. are employees of and holders of equity in Nodality Inc.
- Issue online: 18 APR 2012
- Version of Record online: 14 FEB 2012
- Manuscript Accepted: 17 DEC 2011
- Manuscript Revised: 6 DEC 2011
- Manuscript Received: 30 SEP 2011
- cell signaling;
- acute myeloid leukemia;
- multiparameter analysis;
- flow cytometry;
- single cell network profiling;
- specimen source
Single cell network profiling (SCNP) is used to simultaneously measure the effects of modulators on signaling networks at the single cell level. SCNP-based biomarker assays predictive of response to induction therapy and relapse risk in acute myeloid leukemia (AML) patients are being developed. Such assays have typically used bone marrow (BM) as the sample source of blasts. Because circulating peripheral blasts are detectable in ∼65% of AML patients and peripheral blood (PB) sampling is less invasive than BM sampling, this study was performed to assess the effect of sample source on AML blasts signaling as measured in SCNP assay.
SCNP using multiparametric flow cytometry was used to evaluate the activation state of intracellular signaling molecules in leukemic blasts under basal conditions and after treatment with modulators in 46 pairs of BM mononuclear cells/PB mononuclear cells. The relationship between readouts of modulated intracellular proteins (“nodes”) was measured using linear regression, Bland-Altman method, and Lin's concordance correlation coefficient.
The majority (156/161) of signaling nodes show strong correlations between paired PB and BM samples independently from the statistical method used. Notable exceptions were two PB samples with almost undetectable levels of circulating blasts compared with paired BM samples.
Our results demonstrate that specimen source (BM or PB) does not significantly affect proteomic signaling in patients with AML and circulating blasts. The ability to use PB as a sample source will facilitate the monitoring of cellular signaling effects following administration of targeted therapies and at time points when BM aspirates are not clinically justifiable. © 2012 International Clinical Cytometry Society