How to cite this article: Simon Á, Bagoly Z, Hevessy Z, Csáthy L, Katona E, Vereb G, Ujfalusi A, Szerafin L, Muszbek L, Kappelmayer J. Expression of coagulation factor XIII subunit A in acute promyelocytic leukemia. Cytometry Part B 2012; 82B: 209–216.
Expression of coagulation factor XIII subunit A in acute promyelocytic leukemia†
Article first published online: 26 APR 2012
Copyright © 2012 International Clinical Cytometry Society
Cytometry Part B: Clinical Cytometry
Volume 82B, Issue 4, pages 209–216, July 2012
How to Cite
Simon, Á., Bagoly, Z., Hevessy, Z., Csáthy, L., Katona, É., Vereb, G., Ujfalusi, A., Szerafin, L., Muszbek, L. and Kappelmayer, J. (2012), Expression of coagulation factor XIII subunit A in acute promyelocytic leukemia. Cytometry, 82B: 209–216. doi: 10.1002/cyto.b.21019
- Issue published online: 21 JUN 2012
- Article first published online: 26 APR 2012
- Accepted manuscript online: 20 MAR 2012 11:04AM EST
- Manuscript Accepted: 8 MAR 2012
- Manuscript Revised: 5 MAR 2012
- Manuscript Received: 9 SEP 2011
- OTKA. Grant Number: K-75199
- OTKA-NKTH. Grant Number: NI-69238
- The János Bolyai Research Scholarship of the Hungarian Academy of Sciences
- University of Debrecen, MHSC; Grant number Mec-1/2011 and Lajos Szodoray Prize
- New Hungary Development Plan, co-financed by the European Social Fund. Grant Numbers: TÁMOP 4.2.1./B-09/1/KONV-2010-0007, TÁMOP 4.2.2./B-10/1-2010-0024
- coagulation factor XIII;
- acute promyelocytic leukemia;
- flow cytometry
Leukemic cells often express markers, which are not characteristic of their particular cell lineage. In this study, we identified the “A” subunit of coagulation factor XIII (FXIII-A) in leukemic promyelocytes in de novo AML M3 cases. The cytoplasmic presence of factor XIII-A has previously been shown only in platelets/megakaryocytes and monocytes/macrophages. Furthermore, more recently we described the presence of FXIII-A in leukemic lymphoblasts.
We studied 14 patients with this rare type of acute leukemia in a period of 4 years and investigated their bone marrow samples by 3-color flow cytometry upon diagnosis, mainly focusing on FXIII-A expression of leukemic cells. We detected FXIII-A also by ELISA, Western-blot, and confocal laser scanning microscopy.
This was a homogenous group of AML M3 patients with translocation t(15;17)(q22;q21) detected by fluorescence in situ hybridization (FISH). In 10 out of 14 samples, FXIII-A was detectable by flow cytometry and was coexpressed with markers characteristic for leukemic promyleocytes (CD45dim/CD13+/CD33+/CD117+/cyMPO+ and HLA-DR-/CD34−/CD14−/CD15−). Staining for the markers GPIIb and GPIX were negative, and FXIII-A was identified in the cytoplasm of the cells by confocal microscopy in a relatively high quantity, as measured by ELISA. By Western blot analysis we could identify FXIII-A in the native 82 kDa form and in cleaved forms corresponding to cleavage products observed when purified FXIII-A was treated by human neutrophil elastase.
This novel expression site of FXIII-A in AML M3 can be considered as a leukemia associated immunophenotype and may have pathophysiological significance. © 2012 International Clinical Cytometry Society