How to cite this article: Song JY, Filie AC, Venzon D, Stetler-Stevenson M, Yuan CM. Flow cytometry increases the sensitivity of detection of leukemia and lymphoma cells in bronchoalveolar lavage specimens Cytometry Part B 2012; 82B: 305–312.
Version of Record online: 26 JUL 2012
Published 2012 Wiley Periodicals, Inc.
Cytometry Part B: Clinical Cytometry
Volume 82B, Issue 5, pages 305–312, September 2012
How to Cite
Song, J. Y., Filie, A. C., Venzon, D., Stetler-Stevenson, M. and Yuan, C. M. (2012), Flow cytometry increases the sensitivity of detection of leukemia and lymphoma cells in bronchoalveolar lavage specimens . Cytometry, 82B: 305–312. doi: 10.1002/cyto.b.21033
This article is a US government work and, as such, is in the public domain in the United States of America.
- Issue online: 6 SEP 2012
- Version of Record online: 26 JUL 2012
- Manuscript Accepted: 31 MAY 2012
- Manuscript Revised: 8 MAY 2012
- Manuscript Received: 7 DEC 2011
- Intramural Research Program of the NIH, NCI
- bronchoalveolar lavage;
- flow cytometry;
- hematolymphoid neoplasm
Recent studies have definitively determined that flow cytometry (FC) is significantly more sensitive than cytomorphology (CM) in detection of hematolymphoid neoplasms (HLNs). However, its utility in paucicellular bronchoalveolar lavage (BAL) specimens has not been established.
FC was performed on BAL specimens submitted from 44 patients with a prior diagnosis of HLN. Panels chosen were based upon cellularity of specimen and patient history. FC results were compared with concurrent CM evaluations.
All 44 BALs were deemed satisfactory for FC and yielded informative results that assisted in diagnosis. Diagnoses included 22/44 B-cell neoplasms, 16/44 T-cell neoplasms, four/44 myeloid neoplasms, and two/44 plasma cell neoplasms. Overall concordance was demonstrated between FC and CM in 77% (34/44) of cases. In nine/44 cases (20%), one technique (FC or CM) clearly detected malignant cells when the other did not. FC was more sensitive than CM in detecting a HLN in eight/nine discordant cases. In only one case (one/44, 2%) were malignant HLN cells suspected by CM, but not identified by FC (one/44, 2%).
We demonstrate, in the largest series published to date, that FC can be performed on BAL specimens. FC is indicated in evaluation of BAL for HLN and improves sensitivity of detection of HLN over CM alone. An integrated FC and CM approach is superior to either technique alone in diagnostic evaluation of BAL. Published 2012 Wiley Periodicals, Inc.