Inter-laboratory assessment of flow cytometric monocyte HLA-DR expression in clinical samples

Authors

  • Julie Demaret,

    1. Hospices Civils de Lyon, Hôpital Edouard Herriot, Laboratoire d'Immunologie, Lyon F-69003, France
    2. EAM 4174, HCL-Université Claude Bernard Lyon 1, Lyon, France
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    • Julie Demaret and Alexandre Walencik contributed equally to the work.

  • Alexandre Walencik,

    1. Centre Hospitalo-Universitaire Albert Michallon, Laboratoire d'immunologie, Grenoble, France
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    • Julie Demaret and Alexandre Walencik contributed equally to the work.

  • Marie-Christine Jacob,

    1. Centre Hospitalo-Universitaire Albert Michallon, Laboratoire d'immunologie, Grenoble, France
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  • Jean-François Timsit,

    1. Université Grenoble 1, UFR Santé, Albert Michallon Hospital, Medical ICU, Grenoble, France
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  • Fabienne Venet,

    1. Hospices Civils de Lyon, Hôpital Edouard Herriot, Laboratoire d'Immunologie, Lyon F-69003, France
    2. EAM 4174, HCL-Université Claude Bernard Lyon 1, Lyon, France
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  • Alain Lepape,

    1. EAM 4174, HCL-Université Claude Bernard Lyon 1, Lyon, France
    2. Hospices Civils de Lyon, CH Lyon-Sud, Réanimation, Lyon, France
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  • Guillaume Monneret

    Corresponding author
    1. Hospices Civils de Lyon, Hôpital Edouard Herriot, Laboratoire d'Immunologie, Lyon F-69003, France
    2. EAM 4174, HCL-Université Claude Bernard Lyon 1, Lyon, France
    • Immunology Laboratory, Hopital E. Herriot, Hospices Civils de Lyon, 5 place d'Arsonval, 69437 Lyon Cedex 03, France
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  • How to cite this article: Demaret J, Walencik A, Jacob M, Timsit J, Venet F, Lepape A, Monneret G. Inter-laboratory Assessment of Flow Cytometric Monocyte HLA-DR Expression in Clinical Samples. Cytometry Part B 2013; 84B: 59–62.

Abstract

Background:

Diminished expression of human leukocyte antigen DR on circulating monocytes (mHLA-DR) is a reliable indicator of immunosuppression in critically ill patients, predictive of both adverse outcome and septic complications. The objective of the present work was to test, in an inter-laboratory clinical study, a standardized protocol for mHLA-DR measurement by flow cytometry.

Methods:

mHLA-DR was assessed in fresh whole blood according to a standardized staining protocol. Cells were analyzed on different flow cytometers (FC500, Navios, FACS Canto II) in different laboratories (Lyon and Grenoble). Results were expressed as numbers of antibodies bound per cell (AB/C).

Results:

Correlations between results were excellent (Pearson and interclass correlation coefficients > 0.98). Coefficients of variations for intra-assay precision ranged from 1.9 to 3.2%. Conclusion: The present report highlights the robustness of this standardized flow cytometric protocol for mHLA-DR measurement in multicentric clinical studies. © 2012 International Clinical Cytometry Society

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