How to cite this article: Wagner TA, Lin C-H, Tobin NH, Côté HCF, Sloan DD, Jerome KR, Frenkel LM. Quantification of mitochondrial toxicity in HIV-infected individuals by quantitative pcr compared to flow cytometry Cytometry Part B 2013; 84B: 55–58.
Quantification of mitochondrial toxicity in HIV-infected individuals by quantitative PCR compared to flow cytometry†
Article first published online: 8 OCT 2012
Copyright © 2012 International Clinical Cytometry Society
Cytometry Part B: Clinical Cytometry
Volume 84B, Issue 1, pages 55–58, January/February 2013
How to Cite
Wagner, T. A., Lin, C.-H., Tobin, N. H., Côté, H. C. F., Sloan, D. D., Jerome, K. R. and Frenkel, L. M. (2013), Quantification of mitochondrial toxicity in HIV-infected individuals by quantitative PCR compared to flow cytometry. Cytometry, 84B: 55–58. doi: 10.1002/cyto.b.21045
- Issue published online: 7 JAN 2013
- Article first published online: 8 OCT 2012
- Manuscript Accepted: 28 AUG 2012
- Manuscript Revised: 25 JUL 2012
- Manuscript Received: 2 MAR 2012
- IMPAACT Developmental Virology grant (Frenkel)
- Bristol-Myers Squibb (Frenkel)
- NIH. Grant Number: P30 AI027757
- NIAID, NCI, NIMH, NIDA, NICHD, NHLBI, NCCAM
- antiretroviral therapy;
- mitochondrial toxicity;
- flow cytometry;
- quantitative PCR;
Non-invasive diagnostic assays to evaluate mitochondrial toxicity could have significant clinical utility for HIV-infected individuals on antiretroviral therapy (ART).
This study compared the ratio of mitochondrial to nuclear DNA determined by quantitative polymerase chain reaction (qPCR) to the ratio of mitochondrial to nuclear-encoded proteins by flow cytometry, in peripheral blood mononuclear cells from 73 HIV-infected individuals with and without risk factors for mitochondrial toxicity.
PCR detected similar mitochondrial/nuclear DNA in HIV-infected individuals without a history of ART, and those receiving ART with lipodystrophy, lipoatrophy, or a history of suspected lactic acidosis. However, the ratio was significantly greater in ART-untreated compared to those receiving either stavudine or didanosine. In contrast, flow cytometry did not detect any differences in mitochondrial/nuclear protein (Lin et al., Cytometry B 2009;76B:181–190). There was no correlation between the assays (rho = −0.05, P = 0.65).
Assessment of the mitochondrial/nuclear DNA ratio by qPCR performed better than the mitochondrial/nuclear-encoded protein ratio by flow cytometry to detect adverse effects of nucleoside analogs on mitochondria. © 2012 International Clinical Cytometry Society