CD4+CD25+CD127− assessment as a surrogate phenotype for FOXP3+ regulatory T cells in HIV-1 infected viremic and aviremic subjects

Authors

  • Julien Saison,

    1. Hospices Civils de Lyon, Hôpital Edouard Herriot, Laboratoire d'Immunologie, Lyon, F-69003, France
    2. Université de Lyon, Université Claude Bernard Lyon 1, Lyon, F-69008, France
    3. Hospices Civils de Lyon, Hôpital de la Croix Rousse, Service de Maladies Infectieuses et Tropicales, Lyon, F-69004, France
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    • Julien Saison and Julie Demaret contributed equally to this work.

  • Julie Demaret,

    1. Hospices Civils de Lyon, Hôpital Edouard Herriot, Laboratoire d'Immunologie, Lyon, F-69003, France
    2. Université de Lyon, Université Claude Bernard Lyon 1, Lyon, F-69008, France
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    • Julien Saison and Julie Demaret contributed equally to this work.

  • Fabienne Venet,

    1. Hospices Civils de Lyon, Hôpital Edouard Herriot, Laboratoire d'Immunologie, Lyon, F-69003, France
    2. Université de Lyon, Université Claude Bernard Lyon 1, Lyon, F-69008, France
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  • Christian Chidiac,

    1. Université de Lyon, Université Claude Bernard Lyon 1, Lyon, F-69008, France
    2. Hospices Civils de Lyon, Hôpital de la Croix Rousse, Service de Maladies Infectieuses et Tropicales, Lyon, F-69004, France
    3. INSERM, U851, 21 avenue Tony Garnier, Lyon, F-69007, France
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  • Christophe Malcus,

    1. Hospices Civils de Lyon, Hôpital Edouard Herriot, Laboratoire d'Immunologie, Lyon, F-69003, France
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  • Francoise Poitevin-Later,

    1. Hospices Civils de Lyon, Hôpital Edouard Herriot, Laboratoire d'Immunologie, Lyon, F-69003, France
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  • Jean-Claude Tardy,

    1. Hospices Civils de Lyon, Hôpital de la Croix Rousse, Laboratoire de Virologie, Lyon, F-69004, France
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  • Tristan Ferry,

    1. Université de Lyon, Université Claude Bernard Lyon 1, Lyon, F-69008, France
    2. Hospices Civils de Lyon, Hôpital de la Croix Rousse, Service de Maladies Infectieuses et Tropicales, Lyon, F-69004, France
    3. INSERM, U851, 21 avenue Tony Garnier, Lyon, F-69007, France
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  • Guillaume Monneret

    Corresponding author
    1. Hospices Civils de Lyon, Hôpital Edouard Herriot, Laboratoire d'Immunologie, Lyon, F-69003, France
    2. Université de Lyon, Université Claude Bernard Lyon 1, Lyon, F-69008, France
    • Immunology Laboratory, Hopital E.Herriot, Hospices Civils de Lyon, 5 places d'Arsonval, 69437 Lyon Cedex 03, France
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  • How to cite this article: Saison J, Demaret J, Venet F, Chidiac C, Malcus C, Poitevin-Later F, Tardy J, Ferry T, Monneret G. CD4+CD25+CD127− assessment as a surrogate phenotype for FOXP3+ regulatory T cells in HIV-1 infected viremic and aviremic subjects. Cytometry Part B 2012; 84B: 50–54.

Abstract

Background:

Although likely pivotal, the role of regulatory T cells (Tregs) in HIV pathogenesis remains elusive. This can be partly explained by analytical issues regarding their phenotypic identification in clinical studies. Instead of intracellular FOXP3 staining, CD4+CD25+CD127− phenotype has been proposed as an alternative to identify Tregs in clinical samples. However, its use remains controversial in viremic patients. Therefore, the objective of the present study was to assess the correlation between frequencies of CD4+CD25+CD127− and CD4+CD25+FOXP3+ lymphocytes in viremic and matched aviremic HIV-infected patients.

Methods:

Peripheral blood was collected from HIV-1 infected patients. Eleven viremic patients (Viral Load > 40 copies/mL) were matched (age, sex, CD4+ cell number) with 8 aviremic patients under highly active antiretroviral therapy (HAART). Fresh whole blood was immediately stained to analyze by flow cytometry the correlation between CD4+CD25+CD127− and the reference phenotype CD4+CD25+FOXP3+ lymphocytes in the same tube (four color staining CD4/CD25/CD127/FOXP3 for concomitant analysis of cell surface and intracellular markers).

Results:

In both groups, no significant differences were observed when comparing CD4+CD25+CD127− and CD4+CD25+FOXP3+ cell frequencies. In line, a strong correlation between CD4+CD25+CD127− and CD4+CD25+FOXP3+ lymphocyte percentages was observed in the whole patient population (r: 0.948, P < 0.001) or each group separately: aviremic (r: 0.968, P < 0.001), viremic (r: 0.9, P < 0.001). Finally, we found that most CD4+FOXP3+ cells were indeed CD25+CD127−, both in viremic and aviremic groups (88.5% and 90.9%, respectively).

Conclusions:

We observed that CD4+CD25+CD127− phenotype is a good and easy-to-perform surrogate identification strategy for FOXP3+ regulatory T cells in both viremic and aviremic HIV-1-infected subjects. Thus, it represents a useful tool for monitoring Tregs in clinical research studies based on large cohorts of patients prospectively monitored, including HIV-infected subjects. © 2012 International Clinical Cytometry Society

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