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LITERATURE CITED

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    Shanafelt TD, Kay NE, Jenkins G, Call TG, Zent CS, Jelinek DF, Morice WG, Boysen J, Zakko L, Schwager S, et al. B-cell count and survival: Differentiating chronic lymphocytic leukemia from monoclonal B-cell lymphocytosis based on clinical outcome. Blood 2009; 113: 41884196.
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    Molica S, Giannarelli D, Gentile M, Cutrona G, Di Renzo N, Di Raimondo F, Neri A, Federico M, Ferrrarini M, Morabito F. Differentiating chronic lymphocytic leukemia from monoclonal B-lymphocytosis according to clinical outcome: On behalf of the GIMEMA chronic lymphoproliferative diseases working group. Haematologica 2011; 96: 277283.
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    Nieto WG, Almeida J, Teodosio C, Abbasi F, Allgood SD, Connors F, Rachel JM, Ghia P, Lanasa MC, Rawstron AC, et al. Commentary: Comparison of current flow cytometry methods for monoclonal B cell lymphocytosis detection. Cytometry Part B Clin Cytom 2010; 78B: S4S9.
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    Hanson CA, Kurtin PJ, Dogan A. The proposed diagnostic criteria change for chronic lymphocytic leukemia: Unintended consequences? Blood 2009; 113: 64956496.
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    Wood B. 9-Color and 10-color flow cytometry in the clinical laboratory. Arch Pathol Lab Med 2006; 130: 680690.
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    Shapiro HM, Practical Flow Cytometry, 4th ed. Hoboken: John Wiley & Sons, Inc; 2003.
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    Wersto RP, Chrest FJ, Leary JF, Morris C, Stetler-Stevenson MA, Gabrielson E. Doublet discrimination in DNA cell-cycle analysis. Cytometry 2001; 46: 296306.
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    Sutherland DR, Keeney M, Illingworth A. Practical guidelines for the high-sensitivity detection and monitoring of paroxysmal nocturnal hemoglobinuria clones by flow cytometry. Cytometry Part B Clin Cytom 2012; 82B: 195208.
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    Stetler-Stevenson M, Ahmad E, Barnett D, Braylan RC, DiGiuseppe JA, Marti G, Menozzi D, Oldaker TA, Orfao de Matos A, Rabellino E, et al. Clinical Flow Cytometric Analysis of Neoplastic Hematolymphoid Cells; Approved Guideline—Second Edition, Vol. 27. Wayne, Pennsylvania: Clinical Laboratory Standards Institute; 2007. h43A2.
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    Robinson G, Morgan L, Evans M, McDermott S, Pereira S, Wansbrough-Jones M, Griffin G. Effect of type of haematology analyser on CD4 count. Lancet 1992; 340: 485.
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    Karcher H, Böhning D, Downing R, Mashate S, Harms G. Comparison of two alternative methods for CD4+ T-cell determination (Coulter manual CD4 count and CyFlow) against standard dual platform flow cytometry in Uganda. Cytometry Part B Clin Cytom 2006; 70B: 163169.
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    Gratama JW, Kraan J, Keeney M, Granger V, Barnett D. Reduction of variation in T-cell subset enumeration among 55 laboratories using single-platform, three or four-color flow cytometry based on CD45 and SSC-based gating of lymphocytes. Cytometry 2002; 50: 92101.
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    Levering WH, van Wieringen WN, Kraan J, van Beers WA, Sintnicolaas K, van Rhenen DJ, Gratama JW. Flow cytometric lymphocyte subset enumeration: 10 Years of external quality assessment in the Benelux countries. Cytometry Part B Clin Cytom 2008; 74B: 7990.
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    Hultin LE, Chow M, Jamieson BD, O'Gorman MR, Menendez FA, Borowski L, Denny TN, Margolick JB. Comparison of interlaboratory variation in absolute T-cell counts by single-platform and optimized dual-platform methods. Cytometry Part B Clin Cytom 2010; 78B: 194200.
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    Gratama JW, Braakman E, Kraan J, Lankheet P, Levering WH, Van Den Beemd MW, Van Der Schoot CE, Wijermans P, Preijers F. Comparison of single and dual-platform assay formats for CD34+ haematopoietic progenitor cell enumeration. Clin Lab Haematol 1999; 21: 337346.
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    Marinov I, Luxova A, Tkacova V, Gasova Z, Pohlreich D, Cetkovsky P. Comparison of three single platform methods for CD34+ hematopoietic stem cell enumeration by flow cytometry. Clin Lab 2011; 57: 10311035.
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    Levering WH, Preijers FW, van Wieringen WN, Kraan J, van Beers WA, Sintnicolaas K, van Rhenen DJ, Gratama JW. Flow cytometric CD34+ stem cell enumeration: Lessons from nine years' external quality assessment within the Benelux countries. Cytometry Part B Clin Cytom 2007; 72B: 178188.
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    Mekouar H, Mullier F, Dinon Q, Bailly N, Cornet Y, Chatelain B. No effect of lysis solutions on absolute CD19+ lymphocytes count and CD45 index in chronic lymphocytic leukemia. Cytometry Part B Clin Cytom 2011; 80B: 369372.
  • 20
    Hendrickx A, Bossuyt X. Quantification of the leukocyte common antigen (CD45) in mature B-cell malignancies. Cytometry 2001; 46: 336339.
  • 21
    Vire B, David A, Wiestner A. TOSO, the Fcmicro receptor, is highly expressed on chronic lymphocytic leukemia B cells, internalizes upon IgM binding, shuttles to the lysosome, and is downregulated in response to TLR activation. J Immunol 2011; 187: 40404050.