Properties of human blood monocytes. II. Monocytes from healthy adults are highly heterogeneous within and among individuals
Article first published online: 10 DEC 2013
© 2013 Clinical Cytometry Society
Cytometry Part B: Clinical Cytometry
Volume 86, Issue 2, pages 121–134, March 2014
How to Cite
How to cite this article: Properties of Human Blood Monocytes. II. Monocytes from Healthy Adults are Highly Heterogeneous Within and Among Individuals. Cytometry Part B 2014; 86B: 121–134., , , and
- Issue published online: 25 FEB 2014
- Article first published online: 10 DEC 2013
- Manuscript Accepted: 18 OCT 2013
- Manuscript Revised: 17 SEP 2013
- Manuscript Received: 16 JUN 2013
- Office of Naval Research and the Nevada BRIN/INBRE. Grant Number: NIH P20 RR016464
Human blood monocytes are known to include subsets defined by the expression of CD14 and CD16 but otherwise are often assumed to be relatively homogeneous. However, we had observed additional heterogeneity that led us to a more extensive examination of monocytes.
Blood samples from 200 healthy adults without known immunological abnormalities were examined by analysis with a hematology analyzer and by flow cytometry (FCM) to determine leukocyte differential counts, to identify subsets and to measure expression of monocyte-associated molecules.
The estimated cell counts of monocytes, neutrophils, total lymphocytes, and T cells all varied to a similar extent, that is, ±30–35%. The fractions of monocyte subsets defined by CD14 and CD16 or by CD163 expression also varied among individuals. FCM examinations showed that all the monocyte-associated molecules that were examined varied in expression in this increasing order—CD244, CD4, CD38, CD91, CD11b, toll-like receptor 2 (TLR2), TIA-1, CD14 (on CD14Br+ cells), CD86, CD80, HLA-DQ, CD33, and HLA-DR.
Human blood monocytes are heterogeneous among healthy adults with respect to cell counts, subsets, and the levels of expression of monocyte-associated molecules. An increase in the “non-classical” (CD14Lo/Neg/CD16+) monocyte subset or in the expression of CD11b or TLR2 have known diagnostic/prognostic implications. CD244 and CD4 have well-defined functions on lymphocytes but perform unknown activities on monocytes although their expression appears more narrowly controlled. Together, these data suggest that monocytes should be more extensively examined in both clinical and basic contexts. © 2013 International Clinical Cytometry Society