Basophil activation test in the diagnosis and monitoring of mastocytosis patients with wasp venom allergy on immunotherapy
Version of Record online: 29 JAN 2014
© 2013 Clinical Cytometry Society
Cytometry Part B: Clinical Cytometry
Volume 86, Issue 3, pages 183–190, May 2014
How to Cite
How to cite this article: Basophil Activation Test in the Diagnosis and Monitoring of Mastocytosis Patients with Wasp Venom Allergy on Immunotherapy. Cytometry Part B 2014:86B:183–190., , , , .
- Issue online: 9 APR 2014
- Version of Record online: 29 JAN 2014
- Manuscript Accepted: 3 DEC 2013
- Manuscript Revised: 27 NOV 2013
- Manuscript Received: 3 JUL 2013
- basophil activation test;
- wasp venom allergy;
There is need for an accurate diagnostic test in mastocytosis patients with wasp venom allergy (WVA) and monitoring of these patients during immunotherapy (IT). In this study, we aimed to evaluate sensitivity and specificity of the Basophil Activation Test (BAT) as a diagnostic and monitoring test in patients with mastocytosis and WVA.
Seventeen patients with mastocytosis and WVA and six mastocytosis patients without WVA were included. BAT was performed before the start of IT (first visit) and at 6 weeks (second visit) and 1 year (third visit), after reaching the maintenance dose. Of 17 patients included, 11 completed the third visit. In mastocytosis patients with WVA, dose-dependent wasp-venom induced upregulation of CD63 and CD203c expression on basophils was observed compared with mastocytosis patients without WVA. Serum specific IgE, IgG4, and tryptase levels were measured in all patients.
BAT had a sensitivity of 87% and specificity of 100% in diagnosing WVA in mastocytosis patients. Basophil allergen threshold sensitivity with respect to CD63 and CD203c was significantly decreased in the second visit compared with the first visit and increased significantly in the third visit compared with the second visit. Specific IgE levels increased significantly in the second visit compared with first and decreased significantly in the third visit compared with the second. Specific IgG4 levels rose significantly in the second visit compared with the first and on the third visit compared with the second. Tryptase levels did not change significantly during the study.
BAT represents a diagnostic test with 100% specificity in allergic patients with mastocytosis and these patients are better to be monitored for a longer period during IT. © 2013 International Clinical Cytometry Society