GBCFLUX Acute Leukemia Subcommittee: Bacal NS (Hospital Albert Einstein São Paulo and Centro de Hematologia de São Paulo), Beltrame M (Clinics Hospital, Federal University of Parana), Cavalcanti Jr GB (Universidade Federal do Rio Grande do Norte), Costa ES (Universidade Federal do Rio de Janeiro), Cunha FGP (Universidade Estadual de Campinas), Ikoma MRV (Hospital Amaral Carvalho-Jau-São Paulo), Lorand-Metze IGH (Universidade Estadual de Campinas), Malvezzi M (Clinics Hospital, Federal University of Parana), Pimenta G (Lab Diagnósticos da America Rio de Janeiro), Pires IAC (Centro de Hematologia e Hemoterapia de Santa Catarina-HEMOSC), Sales MM (Laboratório de Citometria de Fluxo do Hospital das Clinicas, Faculdade de Medicina Universidade de São Paulo), Sandes AF (Division of Hematology Fleury Group São Paulo and Division of Hematology and Blood Transfusion Medicine, Escola Paulista de Medicina-Universidade Federal de São Paulo), Santos-Silva MC (Universidade de Santa Catarina), Souto EX (Lab Diagnósticos da América - DASA São Paulo), Thiago LS (Brazilian National Cancer Institute Rio de Janeiro), Yamamoto M (Division of Hematology and Blood Transfusion Medicine, Escola Paulista de Medicina-Universidade Federal de São Paulo).
First proposed panels on acute leukemia for four-color immunophenotyping by flow cytometry from the Brazilian group of flow cytometry-GBCFLUX
Article first published online: 19 APR 2014
© 2015 International Clinical Cytometry Society
Cytometry Part B: Clinical Cytometry
Volume 88, Issue 3, pages 194–203, May/June 2015
How to Cite
How to cite this article: First Proposed Panels on Acute Leukemia for Four-Color Immunophenotyping by Flow Cytometry from the Brazilian Group of Flow Cytometry-GBCFLUX. Cytometry Part B 2015;88B:194–203., , , , , , , , , , .
Conflict of Interest: The authors declare that there is no conflict of interest.
- Issue published online: 23 APR 2015
- Article first published online: 19 APR 2014
- Manuscript Accepted: 28 MAR 2014
- Manuscript Revised: 26 MAR 2014
- Manuscript Received: 23 OCT 2013
- flow cytometry;
- acute leukemia panel;
- acute lymphoblastic leukemia;
- acute myeloblastic leukemia;
Multiparameter flow cytometry is a highly sensitive, fast, and specific diagnostic technology with a wide range of applicability in hematology. Although well-established eight-color immunophenotyping panels are already available, most Brazilian clinical laboratories are equipped with four-color flow cytometer facilities. Based on this fact, the Brazilian Group of Flow Cytometry (Grupo Brasileiro de Citometria de Fluxo, GBCFLUX) for standardization of clinical flow cytometry has proposed an antibody panel designed to allow precise diagnosis and characterization of acute leukemia (AL) within resource-restricted areas. Morphological analysis of bone marrow smears, together with the screening panel, is mandatory for the primary identification of AL. The disease-oriented panels proposed here are divided into three levels of recommendations (mandatory, recommendable, and optional) in order to provide an accurate final diagnosis, as well as allow some degree of flexibility based on available local resources and patient-specific needs. The proposed panels will be subsequently validated in an interlaboratory study to evaluate its effectiveness on the diagnosis and classification of AL. (Assoc editor comm. 2). © 2015 International Clinical Cytometry Society