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Examination of CD26/DPPIV, p53, and PTEN expression in thyroid follicular adenoma

Authors

  • Yasuyuki Miyake Ph.D., C.F.I.A.C.,

    1. Department of Medical Life Science, Kurashiki University of Science and the Arts, Okayama, Japan
    2. Kake Institute of Cytopathology, Okayama, Japan
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  • Yatsuki Aratake Ph.D., C.M.I.A.C.,

    1. Department of Medical Life Science, Kurashiki University of Science and the Arts, Okayama, Japan
    2. Kake Institute of Cytopathology, Okayama, Japan
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  • Takuya Sakaguchi Ph.D.,

    1. Department of Medical Life Science, Kurashiki University of Science and the Arts, Okayama, Japan
    2. Kake Institute of Cytopathology, Okayama, Japan
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  • Kazuaki Kiyoya M.A., C.T., I.A.C.,

    1. Koga General Hospital, Miyazaki, Japan
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  • Tadanobu Kuribayashi M.D., Ph.D.,

    1. Koga General Hospital, Miyazaki, Japan
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  • Kousuke Marutsuka M.D., Ph.D.,

    1. Department of pathology, Faculty of Medicine University of Miyazaki, Miyazaki, Japan
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  • Eiji Ohno Ph.D., C.M.I.A.C.

    Corresponding author
    1. Department of Medical Life Science, Kurashiki University of Science and the Arts, Okayama, Japan
    2. Kake Institute of Cytopathology, Okayama, Japan
    • Department of Medical Life Science, Kurashiki University of Science and Arts, 2640Nishinoura, Tsurajima-cho, Kurashiki-shi, Okayama 712-8505, Japan
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Abstract

Tumor cytology has proven to be inadequate for precise diagnosis of thyroid follicular adenoma. This suggests the need for a molecular approach for its diagnosis.

Expression of CD26/DPPIV (dipeptidyl peptidas IV), p53, and PTEN was analyzed in smears or sections obtained from 19 patients with histologically proven thyroid follicular adenoma. Papanicolaou staining, CD26/DPPIV activity staining, and HE staining were performed and the specimens were observed morphologically. Immunohistochemical analysis using antibodies against p53 and PTEN was performed. Genetic mutation of PTEN exons was performed using the laser capture microdissection method.

The nuclear area of the CD26/DPPIV-positive cells was significantly larger than that of the CD26/DPPIV-negative cells. p53 expression was not observed any specimen. PTEN expression was observed in 18 of 19 cases. DNA sequence analysis did not reveal mutations in exons 5–9 of PTEN in the immunohistochemically PTEN-negative case.

In accordance with our previous reports, we found that observation of concomitant CD26-positive and PTEN-negative status in cases of follicular adenoma suggests a state close to follicular carcinoma or progression to cancer, thus warranting careful follow-up. Diagn. Cytopathol. 2012. © 2011 Wiley Periodicals, Inc.

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