Anti-diabetic effect of standardized extract of Potentilla discolor Bunge and identification of its active components
Article first published online: 19 NOV 2009
© 2009 Wiley-Liss, Inc.
Drug Development Research
Volume 71, Issue 2, pages 127–132, April 2010
How to Cite
Yang, J., Chen, H., Zhang, L., Wang, Q. and Lai, M.-X. (2010), Anti-diabetic effect of standardized extract of Potentilla discolor Bunge and identification of its active components. Drug Dev. Res., 71: 127–132. doi: 10.1002/ddr.20340
- Issue published online: 20 APR 2010
- Article first published online: 19 NOV 2009
- Manuscript Accepted: 13 SEP 2009
- Manuscript Received: 14 JUL 2009
- Potentilla discolor;
- glycogen phosphorylase
Potentilla discolor Bunge, a Chinese folk medicine, has been used for the treatment of diabetes for many years. Unfortunately, little information is available on its bioactive components and mechanism of action. We report the anti-diabetic effect of P. discolor on normal and alloxan-induced diabetic mice, as well as the possible constituents and mechanism responsible for this activity. We found that the standardized extract of P. discolor (EPD) had little effect on the glucose levels of normal mice, while a dose-dependent hypoglycemic effect was observed in diabetic mice. Glucose tolerance test data indicated that there was a significantly higher rate of glucose disposal after EPD treatment. Phytochemical characterization indicated that the major components of EPD were triterpenes and flavonoids that could inhibit the activity of glycogen phosphorylase (GP) in vitro. Tormentic acid, asiatic acid, and potengriffioside A exhibited inhibitory effects on GP (IC50=90.6, 65.4, and 85.9 µM, respectively). The inhibition on GP may be one molecular mechanism through which EPD ameliorates hyperglycemia. These results suggest P. discolor extract may offer an alternate treatment for diabetes. Drug Dev Res, 2009. © 2009 Wiley-Liss, Inc.