Research Article

Influence of diabetic metabolic state on fracture healing in spontaneously diabetic rats

  1. Niels Follak1,*,
  2. Ingrid Klöting2,
  3. Harry Merk1

Article first published online: 3 FEB 2005

DOI: 10.1002/dmrr.537

Issue

Diabetes/Metabolism Research and Reviews

Diabetes/Metabolism Research and Reviews

Volume 21, Issue 3, pages 288–296, May/June 2005

Additional Information

How to Cite

Follak, N., Klöting, I. and Merk, H. (2005), Influence of diabetic metabolic state on fracture healing in spontaneously diabetic rats. Diabetes/Metabolism Research and Reviews, 21: 288–296. doi: 10.1002/dmrr.537

Author Information

  1. 1

    Orthopedic Research Laboratories, Department of Orthopedic Surgery, Ernst Moritz Arndt University, Greifswald, Germany

  2. 2

    Institute for Pathophysiology, Division of Laboratory Animal Science, Karlsburg, Ernst Moritz Arndt University, Greifswald, Germany

*Orthopedic Research Laboratories, Department of Orthopedic Surgery, Ernst Moritz Arndt University, F. Sauerbruch Str., 17487 Greifswald, Germany.

Publication History

  1. Issue published online: 28 APR 2005
  2. Article first published online: 3 FEB 2005
  3. Manuscript Received: 22 JUL 2003

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (407K)

Keywords:

  • spontaneously diabetic rats;
  • fracture healing;
  • callus histomorphometry;
  • diabetes;
  • bone biomechanics

Abstract

Background

Type 1 diabetes mellitus (DM) has been shown to alter the properties of bone and impair fracture healing in both humans and animals. The objective of this study was to examine changes in the histomorphometrical, histological and mechanical parameters of bone and remodeling during fracture healing, depending on the diabetic metabolic state in spontaneously diabetic BB/O(ttawa)K(arlsburg) rats, a rat strain that represents a close homology to DM in man.

Methods

On the basis of blood glucose values at the time of surgery, postoperative blood glucose course and postoperative insulin requirements, 90 spontaneously diabetic BB/OK rats were divided into groups with well-compensated or poorly compensated metabolic state. Forty-five LEW.1A rats served as the normoglycemic controls. The femurs were fractured in a standardized procedure and then allowed to heal for 2, 4 and 6 weeks.

Results

Our study showed that, in terms of bone histomorphometry, within the first 4 weeks after fracture, severe mineralization disorders occurred exclusively in the rats with poorly compensated diabetic metabolic states with a significant decrease of all fluorochrome-based parameters of mineralization, apposition, formation and timing of mineralization, as well as a delay in cellular differentiation and significantly decreased values of biomechanical properties in comparison to the spontaneously diabetic rats with well-compensated metabolic states and to the control rats.

Conclusions

With a controlled insulin therapy and a resulting well-compensated diabetic metabolic state, mineralization and cellular differentiation disorders, as well as the decreased values of biomechanical properties in the fracture repair in the poorly compensated diabetic metabolic state with very severe hyperglycemia can be avoided. Copyright © 2005 John Wiley & Sons, Ltd.

View Full Article (HTML) Get PDF (407K)