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A possible association of Pro12Ala polymorphism in peroxisome proliferator–activated receptor γ2 gene with obesity in native Javanese in Indonesia

Authors

  • C. W. Danawati,

    1. Division of Internal and Geriatric Medicine, Department of Development and Aging, Faculty of Medical Science, Kobe University Graduate School of Medicine, Kobe, Japan
    2. Department of Internal Medicine, Gadjah Mada University, School of Medicine, Yogyakarta, Indonesia
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  • M. Nagata,

    Corresponding author
    1. Division of Internal and Geriatric Medicine, Department of Development and Aging, Faculty of Medical Science, Kobe University Graduate School of Medicine, Kobe, Japan
    • Division of Internal and Geriatric Medicine, Department of Development and Aging, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-Cho, Chuo-ku, Kobe, Japan, 650-0017.
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  • H. Moriyama,

    1. Division of Internal and Geriatric Medicine, Department of Development and Aging, Faculty of Medical Science, Kobe University Graduate School of Medicine, Kobe, Japan
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  • K. Hara,

    1. Division of Internal and Geriatric Medicine, Department of Development and Aging, Faculty of Medical Science, Kobe University Graduate School of Medicine, Kobe, Japan
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  • H. Yasuda,

    1. Division of Internal and Geriatric Medicine, Department of Development and Aging, Faculty of Medical Science, Kobe University Graduate School of Medicine, Kobe, Japan
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  • M. Nakayama,

    1. Division of Internal and Geriatric Medicine, Department of Development and Aging, Faculty of Medical Science, Kobe University Graduate School of Medicine, Kobe, Japan
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  • R. Kotani,

    1. Division of Internal and Geriatric Medicine, Department of Development and Aging, Faculty of Medical Science, Kobe University Graduate School of Medicine, Kobe, Japan
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  • K. Yamada,

    1. Division of Internal and Geriatric Medicine, Department of Development and Aging, Faculty of Medical Science, Kobe University Graduate School of Medicine, Kobe, Japan
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  • M. Sakata,

    1. Division of Internal and Geriatric Medicine, Department of Development and Aging, Faculty of Medical Science, Kobe University Graduate School of Medicine, Kobe, Japan
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  • M. Kurohara,

    1. Division of Internal and Geriatric Medicine, Department of Development and Aging, Faculty of Medical Science, Kobe University Graduate School of Medicine, Kobe, Japan
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  • P. Wiyono,

    1. Department of Internal Medicine, Gadjah Mada University, School of Medicine, Yogyakarta, Indonesia
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  • H. Asdie,

    1. Department of Internal Medicine, Gadjah Mada University, School of Medicine, Yogyakarta, Indonesia
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  • M. Sakaue,

    1. The Laboratory of the Environment for Life and Living, School of Human Science and Environment, University of Hyogo, Japan
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  • H. Taniguchi,

    1. Department of Metabolism and Community Health Science, Faculty of Health Sciences, Kobe University School of Medicine, Kobe, Japan
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  • K. Yokono

    1. Division of Internal and Geriatric Medicine, Department of Development and Aging, Faculty of Medical Science, Kobe University Graduate School of Medicine, Kobe, Japan
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Abstract

Background

Peroxisome proliferators–activated receptor γ (PPAR γ) is a nuclear hormone receptor that serves as a master regulator of adipocytes-specific genes contributing to adipocytes differentiation, susceptibility to obesity, and insulin sensitivity. The substitution of proline to alanine at codon 12 of the PPAR γ2 gene (Pro12Ala polymorphism) is most frequently identified and the associations with diabetes, obesity, and other clinical parameters have been reported and discussed in several ethnic groups. Among native Javanese ethnicity, however, there is no report about this polymorphism.

Aims and methods

Aims of this study were to estimate the allele frequency of the Pro12Ala polymorphism of PPAR γ2 gene among native Javanese in Indonesia and to investigate the relationship between this polymorphism and obesity or diabetes. This study included 540 native Javanese subjects consisting of 337 diabetic patients and 203 normal glucose tolerance subjects. Both groups included totally 160 obese subjects (body mass index ≥ 25 kg/m2). PCR-restriction fragment length polymorphism was used for the genotype determination.

Results

The allele frequency of Pro12Ala polymorphism in PPAR γ2 gene among native Javanese is lower than that in other ethnic groups. No association is seen between the Pro12Ala and diabetes (0.01 vs 0.017%, p = 0.404), a trend of the higher BMI was observed in Pro12Ala carriers in nondiabetic subjects, although this association is limited by small numbers.

Conclusion

In this study, no association is seen between the Pro12Ala polymorphism in PPAR γ2 gene and diabetes; a weak association with obesity is seen. Copyright © 2005 John Wiley & Sons, Ltd.

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