Research Article

HUVECs from newborns with a strong family history of diabetes show diminished ROS synthesis in the presence of high glucose concentrations

  1. Noé Alvarado-Vásquez1,
  2. Araceli Páez2,
  3. Estrella Zapata2,
  4. Susana Alcázar-Leyva3,
  5. Edgar Zenteno4,
  6. Felipe Massó2,
  7. Luis F. Montaño4,*

Article first published online: 30 JUN 2006

DOI: 10.1002/dmrr.665

Issue

Diabetes/Metabolism Research and Reviews

Diabetes/Metabolism Research and Reviews

Volume 23, Issue 1, pages 71–80, January 2007

Additional Information

How to Cite

Alvarado-Vásquez, N., Páez, A., Zapata, E., Alcázar-Leyva, S., Zenteno, E., Massó, F. and Montaño, L. F. (2007), HUVECs from newborns with a strong family history of diabetes show diminished ROS synthesis in the presence of high glucose concentrations. Diabetes Metab. Res. Rev., 23: 71–80. doi: 10.1002/dmrr.665

Author Information

  1. 1

    Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, 14080, México

  2. 2

    Departamento de Biología Celular, Instituto Nacional de Cardiología ‘Ignacio ‘Chávez’, 14080, México

  3. 3

    Instituto de Investigaciones Científicas Hans-Selye, A.C., 76 000, Querétaro, Qro., México

  4. 4

    Laboratorio de Inmunología, Departamento de Bioquímica, Facultad de Medicina UNAM, 04510, México

*Laboratorio de Inmunología, Departamento de Bioquímica, Facultad de Medicina UNAM, A.P. 70-159, C.P. 04510, México.

Publication History

  1. Issue published online: 28 DEC 2006
  2. Article first published online: 30 JUN 2006
  3. Manuscript Accepted: 24 MAY 2006
  4. Manuscript Revised: 11 MAY 2006
  5. Manuscript Received: 8 FEB 2006

Funded by

  • Instituto Científico Pfizer-Mexico. Grant Number: SALUD-2002-COI-7630
  • PAPIIT-UNAM. Grant Number: IN224906-2

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (351K)

Keywords:

  • endothelium;
  • glucose;
  • type 2 diabetes mellitus;
  • superoxide;
  • reactive oxygen species;
  • oxidative stress

Abstract

Background

A family history of type 2 diabetes mellitus (DM) increases the probability to develop DM and endothelial dysfunction. The probable mechanism involves augmented reactive oxygen species (ROS) synthesis. The aim of this study was to evaluate the synthesis of ROS in human umbilical vein endothelial cells (HUVECs) obtained from healthy newborns with (experimental) and without (control) a strong family history of type 2 DM, exposed to different glucose concentrations.

Methods

HUVECs were exposed to various glucose concentrations for 24 and 48 h periods, before cell proliferation, mitochondrial activity, and mitochondrial membrane potential were determined. Intracellular ROS synthesis in the presence or absence of the mitochondrial uncoupler CCCP, cytochalasin B, or diphenyleneiodonium (DPI) was also evaluated.

Results

As opposed to control HUVECs, we found that experimental HUVECs exposed to 30 mmol/L glucose showed a 50% decrease in cell proliferation, a 90% reduction in mitochondrial activity, and a statistically significant inhibition of ROS synthesis in the presence of CCCP or cytochalasin B; DPI had no effect.

Conclusions

Our results suggest that mitochondria and NAD(P)H-oxidase from HUVECs obtained from healthy newborns with a family history of DM have an innate deficient response to high glucose concentrations. Copyright © 2006 John Wiley & Sons, Ltd.

View Full Article (HTML) Get PDF (351K)