Research Article

Serum protein patterns in newly diagnosed type 2 diabetes mellitus—influence of diabetic environment and family history of diabetes

  1. Tea Sundsten1,*,
  2. Claes-Göran Östenson2,
  3. Peter Bergsten1

Article first published online: 29 OCT 2007

DOI: 10.1002/dmrr.789

Issue

Diabetes/Metabolism Research and Reviews

Diabetes/Metabolism Research and Reviews

Volume 24, Issue 2, pages 148–154, February 2008

Additional Information

How to Cite

Sundsten, T., Östenson, C.-G. and Bergsten, P. (2008), Serum protein patterns in newly diagnosed type 2 diabetes mellitus—influence of diabetic environment and family history of diabetes. Diabetes/Metabolism Research and Reviews, 24: 148–154. doi: 10.1002/dmrr.789

Author Information

  1. 1

    Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden

  2. 2

    Department of Molecular Medicine and Surgery, Endocrine and Diabetes Unit, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden

*Department of Medical Cell Biology, Uppsala University, Box 571, SE-751 23 Uppsala, Sweden.

Publication History

  1. Issue published online: 28 JAN 2008
  2. Article first published online: 29 OCT 2007
  3. Manuscript Accepted: 2 SEP 2007
  4. Manuscript Revised: 31 JUL 2007
  5. Manuscript Received: 28 MAY 2007

Funded by

  • Swedish Medical Research Council. Grant Numbers: 72X-14019, 19X-00034
  • European Foundation for the Study of Diabetes, Swedish Foundation for Strategic Research, Swedish Diabetes Association, Swedish Medical Association, Novo Nordisk Foundation
  • Göran Gustafsson Foundation
  • Marcus and Amalia Wallenberg Foundation
  • Family Ernfors Fund
  • Novo Nordisk, Scandinavia
  • GlaxoSmithKline, Sweden

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Keywords:

  • SELDI-TOF MS;
  • type 2 diabetes mellitus;
  • human serum protein profiling;
  • albumin;
  • apolipoprotein C3;
  • transthyretin

Abstract

Background

Individuals with normal glucose tolerance (NGT) and type 2 diabetes mellitus (T2DM) represent heterogeneous groups with differences in β-cell function and genetic background. The aim of the present study was to compare serum protein profiles of NGT and T2DM individuals and determine the influence of the genetic background versus diabetic environment on differentially displayed proteins.

Methods

Surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) was used to compare serum protein profiles of NGT persons and T2DM patients. All participants were from the Stockholm Diabetes Prevention Program (SDPP) cohort. They were selected to have high or low β-cell function (HOMA-β) and family history of type 2 diabetes (FHD) or not.

Results

Eight proteins were found to be elevated and five lowered (p < 0.05) in serum of T2DM patients. In a second comparison, the NGT and T2DM groups were divided into persons with FHD and low HOMA-β and those without FHD and high HOMA-β. Three proteins were rediscovered and interpreted to be different due to genetic background. Two of these were identified as apolipoprotein C3 (apoC3) and albumin. Ten proteins were interpreted to be not related to FHD, and one of these was identified as transthyretin.

Conclusions

Using the SELDI-technique, serum protein profiles of NGT and T2DM persons with differences in β-cell function and FHD were compared. The diabetic environment had a major influence on most of these proteins, while FHD was an important factor for apoC3 and albumin. Copyright © 2007 John Wiley & Sons, Ltd.

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