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Decreased in vivo oxidative stress and decreased platelet activation following metformin treatment in newly diagnosed type 2 diabetic subjects

Authors

  • Gloria Formoso,

    1. Department of Medicine and Aging Sciences, University -G. d'Annunzio, Chieti, Italy
    2. Aging Research Center, Ce.S.I., -G. d'Annunzio- University Foundation, Chieti, Italy
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    • These two authors contributed equally to the manuscript

  • Elena A. De Filippis,

    1. Department of Medicine and Aging Sciences, University -G. d'Annunzio, Chieti, Italy
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    • These two authors contributed equally to the manuscript

  • Noemi Michetti,

    1. Department of Medicine and Aging Sciences, University -G. d'Annunzio, Chieti, Italy
    2. Aging Research Center, Ce.S.I., -G. d'Annunzio- University Foundation, Chieti, Italy
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  • Patrizia Di Fulvio,

    1. Department of Medicine and Aging Sciences, University -G. d'Annunzio, Chieti, Italy
    2. Aging Research Center, Ce.S.I., -G. d'Annunzio- University Foundation, Chieti, Italy
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  • Assunta Pandolfi,

    1. Aging Research Center, Ce.S.I., -G. d'Annunzio- University Foundation, Chieti, Italy
    2. Department of Biomedical Sciences, Chieti-Pescara, Italy
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  • Tonino Bucciarelli,

    1. Aging Research Center, Ce.S.I., -G. d'Annunzio- University Foundation, Chieti, Italy
    2. Department of Drug Sciences University -G. d'Annunzio, Chieti, Italy
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  • Giovanni Ciabattoni,

    1. Aging Research Center, Ce.S.I., -G. d'Annunzio- University Foundation, Chieti, Italy
    2. Department of Drug Sciences University -G. d'Annunzio, Chieti, Italy
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  • Antonio Nicolucci,

    1. Consorzio Mario Negri Sud—S. Maria Imbaro, Italy
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  • Giovanni Davì,

    1. Department of Medicine and Aging Sciences, University -G. d'Annunzio, Chieti, Italy
    2. Aging Research Center, Ce.S.I., -G. d'Annunzio- University Foundation, Chieti, Italy
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  • Agostino Consoli

    Corresponding author
    1. Department of Medicine and Aging Sciences, University -G. d'Annunzio, Chieti, Italy
    2. Aging Research Center, Ce.S.I., -G. d'Annunzio- University Foundation, Chieti, Italy
    • Department of Medicine and Aging Sciences, Edificio CeSI, Room 271, University of Chieti, via dei Vestini, 1, 66100 Chieti, Italy.
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Abstract

Background

In type 2 diabetes, metformin reduces cardiovascular risk beyond the effect of glycaemic control. Since oxidative stress and the consequent enhanced platelet activation contribute to accelerated atherosclerosis in diabetes, we hypothesized that metformin could reduce oxidative stress in this condition.

Methods

We randomized 26 newly diagnosed type 2 diabetic subjects to assume either metformin (M, n = 13) or gliclazide (G, n = 13) for 12 weeks. Drugs were titrated as needed to achieve good glycaemic control. Before and after treatment, we determined blood glucose, insulin, HbA1c, vitamin A and E levels and 8-iso-PGF and 11-dehydro-thromboxane B2 urinary excretion, an in vivo oxidative stress and a thromboxane-dependent platelet activation marker, respectively.

Results

Notwithstanding a comparable improvement in metabolic control, 8-iso-PGF (M from 708 ± 32 to 589 ± 45 pg/mg cr, p < 0.001; G from 646 ± 80 to 665 ± 79, pg/mg cr, p = ns) and 11-dehydro-thromboxane B2 (M from 2190 ± 196 to 1753 ± 150 pg/mg cr, p < 0.05; G from 2048 ± 202 to 1923 ± 223, pg/mg cr, p = ns) urinary excretion decreased after metformin but not after gliclazide treatment. After metformin, vitamin A and E levels significantly increased while they remained unchanged after gliclazide.

Conclusions

These data suggest that metformin could improve oxidative stress, preserve antioxidant function and restrain platelet activation in type 2 diabetes. Copyright © 2007 John Wiley & Sons, Ltd.

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