This article is a US Government work and, as such, is in the Public domain in the United States of America.
Imaging the spatiotemporal organization of neural activity in the developing spinal cord†
Article first published online: 28 MAR 2008
Published © 2008 Wiley Periodicals, Inc.
Special Issue: Dynamic Imaging of the Developing Nervous System
Volume 68, Issue 6, pages 788–803, May 2008
How to Cite
O'Donovan, M. J., Bonnot, A., Mentis, G. Z., Arai, Y., Chub, N., Shneider, N. A. and Wenner, P. (2008), Imaging the spatiotemporal organization of neural activity in the developing spinal cord. Devel Neurobio, 68: 788–803. doi: 10.1002/dneu.20620
- Issue published online: 10 APR 2008
- Article first published online: 28 MAR 2008
- Manuscript Accepted: 31 AUG 2007
- Manuscript Revised: 27 AUG 2007
- Manuscript Received: 19 APR 2007
- National Institutes of Neurological Disorder and Stroke
- spinal cord;
In this review, we discuss the use of imaging to visualize the spatiotemporal organization of network activity in the developing spinal cord of the chick embryo and the neonatal mouse. We describe several different methods for loading ion- and voltage-sensitive dyes into spinal neurons and consider the advantages and limitations of each one. We review work in the chick embryo, suggesting that motoneurons play a critical role in the initiation of each cycle of spontaneous network activity and describe how imaging has been used to identify a class of spinal interneuron that appears to be the avian homolog of mammalian Renshaw cells or 1a-inhibitory interneurons. Imaging of locomotor-like activity in the neonatal mouse revealed a wave-like activation of motoneurons during each cycle of discharge. We discuss the significance of this finding and its implications for understanding how locomotor-like activity is coordinated across different segments of the cord. In the last part of the review, we discuss some of the exciting new prospects for the future. © 2008 Wiley Periodicals, Inc. Develop Neurobiol, 2008