A novel role for anosmin-1 in the adhesion and migration of oligodendrocyte precursors

Authors

  • Ana Bribián,

    1. Grupo de Neurobiología del Desarrollo-GNDe, Hospital Nacional de Parapléjicos, Finca “La Peraleda” s/n, Toledo E-45071, Spain
    Current affiliation:
    1. Department of Cell Biology and Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), University of Barcelona, Barcelona E-08028, Spain
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  • Pedro F. Esteban,

    1. Grupo de Neurobiología del Desarrollo-GNDe, Hospital Nacional de Parapléjicos, Finca “La Peraleda” s/n, Toledo E-45071, Spain
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  • Diego Clemente,

    1. Grupo de Neurobiología del Desarrollo-GNDe, Hospital Nacional de Parapléjicos, Finca “La Peraleda” s/n, Toledo E-45071, Spain
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  • Nadia Soussi-Yanicostas,

    1. INSERM Equipe Avenir, IFR 70, Hôpital de la Salpêtrière 47, Bd de l'Hôpital, Paris F-75651, France
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  • Jean-Léon Thomas,

    1. Biologie des Interactions Neurones/Glie, Unité Mixte de R, echerche INSERM U-711/UPMC, Hôpital de la Salpêtrière 47, Bd de l'Hôpital, Paris F-75013, France
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  • Bernard Zalc,

    1. Biologie des Interactions Neurones/Glie, Unité Mixte de R, echerche INSERM U-711/UPMC, Hôpital de la Salpêtrière 47, Bd de l'Hôpital, Paris F-75013, France
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  • Fernando de Castro

    Corresponding author
    1. Grupo de Neurobiología del Desarrollo-GNDe, Hospital Nacional de Parapléjicos, Finca “La Peraleda” s/n, Toledo E-45071, Spain
    • Grupo de Neurobiología del Desarrollo-GNDe, Hospital Nacional de Parapléjicos, Finca “La Peraleda” s/n, Toledo E-45071, Spain
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Abstract

At embryonic stages of development, oligodendrocyte precursors (OPCs) generated in the preoptic area colonize the entire optic nerve (ON). Different factors controlling migration of ON OPCs have been identified, including secreted growth factors, morphogens and guidance cues, as well as cell adhesion molecules. We have shown previously that the soluble form of the extracellular matrix (ECM) protein anosmin-1, impairs OPC migration induced by FGF-2. In the present work, we show that anosmin-1 is expressed by both migrating OPCs and axons of the retinal ganglion cells in the embryonic ON. In vitro, we observe that OPC migration is strongly impaired by contact with anosmin-1 when used as a substrate and, in contrast to previous results, this effect is independent of FGF-2/FGFR1 signaling. We also show that OPCs preferentially adhere to anosmin-1 when compared with other ECM molecules used as substrates, and that when the endogenous anosmin-1 expressed by OPCs is blocked, OPC adhesion to all the different substrates (including anosmin-1), is significantly reduced. This novel effect of anosmin-1 on cell adhesion is also independent of FGF-2/FGFR1. We finally demonstrate that the blockade of the endogenous anosmin-1 expressed by OPCs impairs their migration. Our data suggest that the endogenous anosmin-1 expressed by OPCs is necessary for the correct adhesion of these cells to the different components of the ECM (including anosmin-1 itself), contributing to the migration of these cells. © 2008 Wiley Periodicals, Inc. Develop Neurobiol 2008

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