Embryonic origins of the mouse superior olivary complex

Authors

  • Glen S. Marrs,

    1. Sensory Neuroscience Research Center, West Virginia University School of Medicine, Morgantown, West Virginia
    2. Department of Otolaryngology, West Virginia University School of Medicine, Morgantown, West Virginia
    Current affiliation:
    1. Department of Biology, Wake Forest University, Winston-Salem, NC
    Search for more papers by this author
    • These authors contributed equally to the work presented.

  • Warren J. Morgan,

    1. Sensory Neuroscience Research Center, West Virginia University School of Medicine, Morgantown, West Virginia
    2. Department of Otolaryngology, West Virginia University School of Medicine, Morgantown, West Virginia
    3. Department of Biochemistry, West Virginia University School of Medicine, Morgantown, West Virginia
    Search for more papers by this author
  • David M. Howell,

    1. Sensory Neuroscience Research Center, West Virginia University School of Medicine, Morgantown, West Virginia
    2. Department of Otolaryngology, West Virginia University School of Medicine, Morgantown, West Virginia
    3. Department of Biochemistry, West Virginia University School of Medicine, Morgantown, West Virginia
    Search for more papers by this author
  • George A. Spirou,

    1. Sensory Neuroscience Research Center, West Virginia University School of Medicine, Morgantown, West Virginia
    2. Department of Otolaryngology, West Virginia University School of Medicine, Morgantown, West Virginia
    3. Department of Physiology and Pharmacology, West Virginia University School of Medicine, Morgantown, West Virginia
    Search for more papers by this author
  • Peter H. Mathers

    Corresponding author
    1. Department of Otolaryngology, West Virginia University School of Medicine, Morgantown, West Virginia
    2. Department of Biochemistry, West Virginia University School of Medicine, Morgantown, West Virginia
    3. Department of Ophthalmology, West Virginia University School of Medicine, Morgantown, West Virginia
    • Sensory Neuroscience Research Center, West Virginia University School of Medicine, Morgantown, West Virginia
    Search for more papers by this author

Correspondence to: P.H. Mathers (pmathers@hsc.wvu.edu).

Abstract

Many areas of the central nervous system are organized into clusters of cell groups, with component cell groups exhibiting diverse but related functions. One such cluster, the superior olivary complex (SOC), is located in the ventral auditory brainstem in mammals. The SOC is an obligatory contact point for most projection neurons of the ventral cochlear nucleus and plays central roles in many aspects of monaural and binaural information processing. Despite their important interrelated functions, little is known about the embryonic origins of SOC nuclei, due in part to a paucity of developmental markers to distinguish individual cell groups. In this report, we present a collection of novel markers for the developing SOC nuclei in mice, including the transcription factors FoxP1, MafB, and Sox2, and the lineage-marking transgenic line En1-Cre. We use these definitive markers to examine the rhombic lip and rhombomeric origins of SOC nuclei and demonstrate that they can serve to uniquely identify SOC nuclei and subnuclei in newborn pups. The markers are also useful in identifying distinct nuclear domains within the presumptive SOC as early as embryonic day (E) 14.5, well before morphological distinction of individual nuclei is evident. These findings indicate that the mediolateral and dorsoventral position of SOC nuclei characteristic of the adult brainstem is established during early neurogenesis. © 2013 Wiley Periodicals, Inc. Develop Neurobiol 73: 384–398, 2013

Ancillary