The analysis of antipsychotic drugs in human matrices using LC-MS(/MS)
Article first published online: 10 MAY 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Drug Testing and Analysis
Volume 4, Issue 6, pages 376–394, June 2012
How to Cite
Saar, E., Beyer, J., Gerostamoulos, D. and Drummer, O. H. (2012), The analysis of antipsychotic drugs in human matrices using LC-MS(/MS). Drug Test Analysis, 4: 376–394. doi: 10.1002/dta.1337
- Issue published online: 27 JUN 2012
- Article first published online: 10 MAY 2012
- Manuscript Revised: 17 FEB 2012
- Manuscript Accepted: 17 FEB 2012
- Manuscript Received: 24 JAN 2012
- antipsychotic drugs;
- method validation
Antipsychotic drugs (APs) are prescribed for a wide range of psychotic illnesses. With more than 35 APs currently available worldwide, this drug class has rapidly gained importance in both clinical and forensic settings. On account of their chemical properties, many APs are present in human specimens at very low concentrations, which complicate their detection using standard gas chromatography–mass spectrometry (GC-MS) procedures that often cannot provide the required sensitivity. Recent advances in liquid chromatography-(tandem) mass spectrometry LC-MS(/MS) technology have enabled accurate detection and quantification of these compounds in various human specimens, indicated by the increasing number of published methods. Method validation has been a particular focus of analytical chemistry in recent times. Recommendations set by several guidance documents are now widely accepted by the toxicology community, as reflected by the guidelines drafted by leading toxicological societies. This review provides a critical review of single-stage and tandem LC-MS procedures for the detection and quantification of APs, with a particular emphasis on appropriate method validation.
The quality of published methods is inconsistent throughout the literature. While the majority of authors incorporate some validation experiments in their respective method development, a large number of published methods lack essential components of method validation, which are considered mandatory according to the guidelines.
If adapting a method for the detection of APs for use in a laboratory, analysts should ensure successful validation experiments for appropriateness and completeness have been conducted, and perform additional experiments when indicated. Copyright © 2012 John Wiley & Sons, Ltd.