A novel flow-injection chemiluminescence method for determination of andrographolide in andrographis tablets
Version of Record online: 19 APR 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Drug Testing and Analysis
Volume 5, Issue 5, pages 340–345, May 2013
How to Cite
Jiang, Z., Hao, Z., Wu, Q., Li, Y., Liu, H. and Yan, L. (2013), A novel flow-injection chemiluminescence method for determination of andrographolide in andrographis tablets. Drug Test Analysis, 5: 340–345. doi: 10.1002/dta.1346
- Issue online: 24 MAY 2013
- Version of Record online: 19 APR 2012
- Manuscript Accepted: 28 FEB 2012
- Manuscript Revised: 8 FEB 2012
- Manuscript Received: 2 SEP 2011
- National Natural Science Foundation of China. Grant Number: 31060193
- Natural Scientific Foundation of Guangxi province, China. Grant Number: GSR0731021
A novel method for determination of andrographolide using flow-injection chemiluminescence (FI-CL) analysis is described in this paper. The chemiluminescence intensity of the solution was enhanced proportionally while the concentration of andrographolide increased. Under the selected experimental conditions, the calibration curve of andrographolide was linear within the range of 0.2 to 35.0 µg mL−1 with a linear equation of ΔI = 23.391x (µg mL−1) + 34.191, R2 = 0.9965. The detection limit (3σ) was 7.42 × 10−2 µg mL−1. At the case of continuous determination of andrographolide, the relative standard deviation (RSD, n = 11) was less than 1.82%. The method has been successfully applied to the determination of andrographis tablets with satisfactory results. Copyright © 2012 John Wiley & Sons, Ltd.