This article was published online on [28 June 2012]. Errors were subsequently identified. This notice is included in the online and print versions to indicate that both have been corrected [12 May 2013].
Effect of Morin on pharmacokinetics of Piracetam in rats, in vitro enzyme kinetics and metabolic stability assay using rapid UPLC method†
Article first published online: 28 JUN 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Drug Testing and Analysis
Volume 5, Issue 7, pages 581–588, July 2013
How to Cite
Sahu, K., Shaharyar, M. and Siddiqui, A. A. (2013), Effect of Morin on pharmacokinetics of Piracetam in rats, in vitro enzyme kinetics and metabolic stability assay using rapid UPLC method. Drug Test Analysis, 5: 581–588. doi: 10.1002/dta.1382
- Issue published online: 8 JUL 2013
- Article first published online: 28 JUN 2012
- Manuscript Accepted: 15 MAY 2012
- Manuscript Revised: 10 MAY 2012
- Manuscript Received: 24 APR 2012
- human liver microsomes;
- in vivo;
- in vitro, UPLC
The aim of this study was to investigate the effect of Morin on the pharmacokinetics of Piracetam in rats, in vitro enzyme kinetics and metabolic stability (high throughput) studies using human liver microsomes in UPLC. For pharmacokinetics studies, male Wistar rats were pretreated with Morin (10 mg/kg) for one week and on the last day, a single dose of Piracetam (50 mg/kg) was given orally. In another group, both Morin and Piracetam were co-administered to evaluate the acute effect of Morin on Piracetam. The control group received oral distilled water for one week and administered with Piracetam on the last day. As Morin is an inhibitor of P- Glycoprotein (P-gp) and CYP 3A, it was anticipated to improve the bioavailability of Piracetam. Amazingly, relative to control, the areas under the concentration time curve and peak plasma concentration of Piracetam were 1.50- and 1.45-fold, respectively, greater in the Morin-pretreated group. However, co-administration of Morin had no significant effect on these parameters. Apart from the aforementioned merits, the results of this study are further confirmed by clinical trials; Piracetam dosages should be adjusted to avoid potential drug interaction when Piracetam is used clinically in combination with Morin and Morin-containing dietary supplements. The in vitro enzyme kinetics were performed to determined km, Vmax & CLins. The in vitro metabolic stability executed for the estimation of metabolic rate constant and half-life of Piracetam. These studies also extrapolate to in vivo intrinsic hepatic clearance (Clint, in vivo) from in vitro intrinsic hepatic clearance (CLint, in vitro). Copyright © 2012 John Wiley & Sons, Ltd.