These authors contributed equally to this work.
Characterization of nucleobases and nucleosides in the fruit of Alpinia oxyphylla collected from different cultivation regions
Article first published online: 14 FEB 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Drug Testing and Analysis
Volume 6, Issue 3, pages 239–245, March 2014
How to Cite
Song, W., Li, Y., Wang, J., Li, Z. and Zhang, J. (2014), Characterization of nucleobases and nucleosides in the fruit of Alpinia oxyphylla collected from different cultivation regions. Drug Test Analysis, 6: 239–245. doi: 10.1002/dta.1462
- Issue published online: 19 MAR 2014
- Article first published online: 14 FEB 2013
- Manuscript Revised: 10 JAN 2013
- Manuscript Accepted: 10 JAN 2013
- Manuscript Received: 19 OCT 2012
- National Science & Technology Pillar Program. Grant Number: 2011BA101B07
- National Natural Science Foundation of Hainan Province. Grant Number: 812189
- Scientific Research of Hainan Medical University. Grant Number: HY2012-006, HY2012-013
- Alpinia oxyphylla;
- cultivation region;
- hierarchical cluster analysis
The fruit of Alpinia oxyphylla, known as Yizhi, Yakuchi and Ikji in Chinese, Japanese, and Korean, respectively, has been utilized as an important drug for the treatment of diarrhea, dyspepsia, spermatorrhea, kidney asthenia and abdominal pain in East Asian traditional medicine for thousands of years. Since the therapeutic effects of A. oxyphylla are attributed to multiple components and nucleobases and nucleosides exhibit various bioactivities, it is necessary to explore the chemical characterization of nucleobases and nucleosides in this herb. Herein, 10 common nucleobases and nucleosides, including cytidine, adenosine, thymidine, inosine, guanosine, 2’-deoxyinosine, guanine, adenine, cytosine, and hypoxanthine, were quantified simultaneously in the fruit of A. oxyphylla collected from different geographical regions. Changes in their contents were discussed, and hierarchical cluster analysis (HCA) was performed to classify all samples on the basis of the contents of the investigated analytes. The results indicated that there was a large variation in the contents of nucleobases and nucleosides among the herbs from different regions, and the samples collected from the same cultivation region were mostly classified in one cluster. The method can be used for comprehensive quality evaluation of A. oxyphylla. Copyright © 2013 John Wiley & Sons, Ltd.