Analytical approaches for the determination of phytocannabinoids and endocannabinoids in human matrices

Authors

  • Natalia Battista,

    Corresponding author
    1. Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy
    2. European Center for Brain Research/Santa Lucia Foundation, Rome, Italy
    • Correspondence to: Natalia Battista, Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Piazza A. Moro 45, 64100 Teramo, Italy. E-mail: nbattista@unite.it; Manuel Sergi, Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Piazza A. Moro 45, 64100 Teramo, Italy. E-mail: msergi@unite.it

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    • Natalia Battista and Manuel Sergi contributed equally to this manuscript and should be considered joint first authors; Dario Compagnone and Mauro Maccarrone should be considered joint last authors.
  • Manuel Sergi,

    1. Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy
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    • Natalia Battista and Manuel Sergi contributed equally to this manuscript and should be considered joint first authors; Dario Compagnone and Mauro Maccarrone should be considered joint last authors.
  • Camilla Montesano,

    1. Department of Chemistry, Sapienza University of Rome, Rome, Italy
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  • Sabino Napoletano,

    1. Department of Chemistry, Sapienza University of Rome, Rome, Italy
    2. Department of Public Safety, Ministry of Interior, Forensic Service/GIPS, Ancona, Italy
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  • Dario Compagnone,

    1. Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy
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  • Mauro Maccarrone

    1. European Center for Brain Research/Santa Lucia Foundation, Rome, Italy
    2. Center of Integrated Research, Campus Bio-Medico University of Rome, Rome, Italy
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Abstract

Over the last two decades, the role played by phytocannabinoids and endocannabinoids in medicine has gained increasing interest in the scientific community. Upon identification of the plant compound Δ9-tetrahydrocannabinol (THC) and of the endogenous substance anandamide (AEA), different methodological approaches and innovative techniques have been developed, in order to evaluate the content of these molecules in various human matrices. In this review, we discuss the analytical methods that are currently used for the identification of phytocannabinoids and endocannabinoids, and we summarize the benefits and limitations of these procedures. Moreover, we provide an overview of the main biological matrices that have been analyzed to date for qualitative detection and quantitative determination of these compounds. Copyright © 2013 John Wiley & Sons, Ltd.

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