A new validated ultra performance liquid chromatographic method for determination of acyclovir
Article first published online: 29 OCT 2010
Copyright © 2010 John Wiley & Sons, Ltd.
Drug Testing and Analysis
Volume 3, Issue 3, pages 187–190, March 2011
How to Cite
Hasan, S., Chander, P., Ali, J., Baboota, S. and Ali, M. (2011), A new validated ultra performance liquid chromatographic method for determination of acyclovir. Drug Test Analysis, 3: 187–190. doi: 10.1002/dta.201
- Issue published online: 18 MAR 2011
- Article first published online: 29 OCT 2010
- Manuscript Accepted: 1 SEP 2010
- Manuscript Revised: 12 AUG 2010
- Manuscript Received: 16 MAY 2010
- method development;
Recently, ultra performance liquid chromatography (UPLC) has proven to be one of the most promising developments in the area of fast chromatographic separations, as it's been true to the objective of reducing analysis time and maintaining good efficiency. The present report describes development and validation of a new, rapid, and sensitive UPLC method with UV detection to quantify acyclovir in bulk sample using a Waters Acquity HSS T-3 (100 × 2.1 mm, 1.8 µm) column maintained at a temperature of 50 °C with a gradient elution consisting of 1.0% triethyl amine in water and acetonitrile at a flow rate of 0.4 mL/min and detected at 254 nm. Method was found to be selective, linear, accurate, and precise as per ICH guidelines. Detection and quantitation limits of drug were 3 and 8 ng/mL, respectively. Copyright © 2010 John Wiley & Sons, Ltd.