Drug Testing and Analysis

Cover image for Drug Testing and Analysis

January 2010

Volume 2, Issue 1

Pages 1–44

  1. Cover Pictures

    1. Top of page
    2. Cover Pictures
    3. Research Articles
    1. Cover Picture

      Article first published online: 23 FEB 2010 | DOI: 10.1002/dta.122

  2. Research Articles

    1. Top of page
    2. Cover Pictures
    3. Research Articles
    1. The use of in vitro technologies coupled with high resolution accurate mass LC-MS for studying drug metabolism in equine drug surveillance (pages 1–10)

      James P. Scarth, Holly A. Spencer, Sarah E. Timbers, Simon C. Hudson and Lynn L. Hillyer

      Article first published online: 3 FEB 2010 | DOI: 10.1002/dta.88

      Thumbnail image of graphical abstract

      Results of experiments to demonstrate the potential use of in vitro methods for studying drug metabolism in equine sport's drug surveillance are presented. Coupled with the use of high-resolution accurate-mass LC-MS, in vitro methods provide a means of reducing, refining and complimenting in vivo experiments.

    2. Development of a high sensitivity bioanalytical method for alprazolam using ultra-perfor-mance liquid chromatography/tandem mass spectrometry (pages 11–18)

      Joanne Mather, Paul D. Rainville, Warren B. Potts III, Norman W. Smith and Robert S. Plumb

      Article first published online: 3 FEB 2010 | DOI: 10.1002/dta.90

      Thumbnail image of graphical abstract

      A rapid, specific, assay was developed for the benzodiapine alprazolam in rat plasma using sub 2 µm particle LC and tandem quadrupole MS/MS. The limit of quantification using protein precipitation was determined to 10 pg/mL, where as the limit of quantification using solid phase extraction was determined to be 1.0 pg/mL. The assay was optimised for throughput and resolution of the analyte of interest from the hydroxy metabolite. During the method development process the plasma matrix signal was monitored, for lipids and other endogenous metabolites, to maximize signal response and minimize ion suppression.

    3. Estimating measurement uncertainty in quantitative methods not based on chromatography for doping control purposes (pages 19–23)

      P. Van Eenoo, P. Van Renterghem, C. H. Dimopoulou, F.T. Delbeke and C. G. Georgakopoulos

      Article first published online: 3 FEB 2010 | DOI: 10.1002/dta.94

      Thumbnail image of graphical abstract

      ISO17025 requires that measurement uncertainty (MU) is estimated and taken into account when compliance of an athlete's sample with the anti-doping regulations is tested. A methodology for the determination and the evaluation of MU in doping control as well as the determination of a decision limit for non-chromatography based methods is presented.

    4. An application of Ag(III) complex chemiluminescence system for the determination of enoxacin in capsule and biological fluid (pages 24–27)

      Peiyun Chen and Hanwen Sun

      Article first published online: 3 FEB 2010 | DOI: 10.1002/dta.104

      Thumbnail image of graphical abstract

      Ag(III) complex chemiluminescence (CL) system was applied for the determination of enoxacin (ENX). The CL conditions of [Ag(HIO6)2]5−[BOND] H2SO4[BOND] ENX systems without any luminescence reagent were investigated and optimized. The proposed method was applied successfully to the determination of the drug in capsule, serum and urine samples.

    5. Spectrophotometric determination of peptic ulcer sulfur-containing drugs in bulk form and in tablets (pages 28–36)

      Gehad G. Mohamed, F. A. Nour El-Dien, Shaban M. Khalil and Aliaa S. M. El-Tantawy

      Article first published online: 3 DEC 2009 | DOI: 10.1002/dta.82

      Thumbnail image of graphical abstract

      Two spectrophotometric procedures base on oxidation with KIO3 or ion-pair formation with dyestuffs are suggested for the determination of rabeprazole (RAB), omeprazole (OMP) and pantoprazole (PAN) in pure form and in different pharmaceutical formulations. The optimum assay conditions were investigated and the recovery of the drugs from their dosage forms ranged from 99.33% to 100.5%. The two methods can be applied successfully for the determination of these drugs in tablets. The results were validated statistically through recovery studies.

    6. Simultaneous determination of metronidazole and spiramycin in bulk powder and in tablets using different spectrophotometric techniques (pages 37–44)

      Fatma I. Khattab, Nesrin K. Ramadan, Maha A. Hegazy and Nermine S. Ghoniem

      Article first published online: 12 JAN 2010 | DOI: 10.1002/dta.83

      Thumbnail image of graphical abstract

      Different spectrophotometric were developed and validated for determination of metronidazole and spiramycin in bulk powder, laboratory prepared mixture and in tablets. These methods are direct, derivative, derivative ratio and mean centering of ratio spectra methods. These methods were statistically compared to a reported HPLC method

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