Metabolism of boldione in humans by mass spectrometric techniques: detection of pseudoendogenous metabolites (pages 834–842)
Xavier de la Torre, Davide Curcio, Cristiana Colamonici, Francesco Molaioni and Francesco Botrè
Article first published online: 20 NOV 2013 | DOI: 10.1002/dta.1567
Boldione is an anabolic androgenic steroid (AAS) related to boldenone, androstenedione and testosterone included in the World Anti-Doping Agency (WADA) list of prohibited substances and methods. Boldione metabolism includes well known boldenone metabolites easily detectable by GC-MS and metabolites identical to the endogenously produced ones, formed after the reduction of the double bound in C1. A method based on gas chromatography coupled to isotope ratio mass spectrometry (GC/C/IRMS) following urine purification by liquid chromatography (HPLC) permitted to confirm both the main synthetic like boldione/boldenone metabolite (17β-hydroxy-5β-androst-1-en-3-one) and boldenone at trace levels and to determine the exogenous origin of metabolites with the same chemical structure of the endogenous ones along a time window overlapping the detection of boldenone and its main metabolite.