Habiboallah Khajehsharifi, Hossein Tavallali, Manzar Shekoohi and Maasumeh Sadeghi
An orthogonal-signal correction-partial least squares method was developed for the simultaneous spectrophotometric determination of orotic acid (OA), creatinine (CRE), and uric acid (UA) in spiked real samples.
Calibration matrices contained 1.74–47.00 of OA, 1.13–33.95 of CRE, and 1.68–28.58 of UA in mg/ml. The number of principal component for OA, CRE, and UA with OSC were 3, 4, and 4. The proposed method was applied for the simultaneous determination of OA, CRE, and UA in real samples.