Green fluorescent protein expression in germ-line transmitted transgenic zebrafish under a stratified epithelial promoter from Keratin8
Article first published online: 27 DEC 2001
Copyright © 2002 Wiley-Liss, Inc.
Volume 223, Issue 2, pages 204–215, February 2002
How to Cite
Gong, Z., Ju, B., Wang, X., He, J., Wan, H., Sudha, P. M. and Yan, T. (2002), Green fluorescent protein expression in germ-line transmitted transgenic zebrafish under a stratified epithelial promoter from Keratin8. Dev. Dyn., 223: 204–215. doi: 10.1002/dvdy.10051
- Issue published online: 30 JAN 2002
- Article first published online: 27 DEC 2001
- Manuscript Accepted: 11 OCT 2001
- Manuscript Received: 5 SEP 2001
- National University of Singapore
- GFP-fusion protein;
- intermediate filaments;
A zebrafish cDNA encoding a novel keratin protein was characterized and named keratin8, or krt8. krt8 expression was initiated at 4.5 hr postfertilization, immediately after the time of zygotic genome activation. The expression is limited to a single layer of envelope cells on the surface of embryos and, in later stages, it also appears in the innermost epithelial layer of the anterior- and posteriormost portions of the digestive tract. In adult, its expression was limited to the surface layer of stratified epithelial tissues, including skin epidermis and epithelia of mouth, pharynx, esophagus, and rectum but not in the gastral and intestinal epithelia. By using a 2.2-kb promoter from krt8, several stable green fluorescent protein (gfp) transgenic zebrafish lines were established. All of these transgenic lines displayed GFP expression in tissues mentioned above except for the rectum; therefore, the pattern of transgenic GFP expression is essentially identical to that of the endogenous krt8 mRNAs. krt8-GFP fusion protein was also expressed in zebrafish embryos under a ubiquitous promoter, and the fusion protein was capable of assembling into intermediate filaments only in the epithelia that normally expressed krt8 mRNAs, indicating the specificity of keratin assembly in vivo. © 2002 Wiley-Liss, Inc.