Mouse dystrophin enhancer preferentially targets lacZ expression in skeletal and cardiac muscle
Version of Record online: 3 APR 2002
Copyright © 2002 Wiley-Liss, Inc.
Volume 224, Issue 1, pages 30–38, May 2002
How to Cite
Marshall, P., Chartrand, N., De Repentigny, Y., Kothary, R., Pelletier, L., Mueller, R. and Worton, R. G. (2002), Mouse dystrophin enhancer preferentially targets lacZ expression in skeletal and cardiac muscle. Dev. Dyn., 224: 30–38. doi: 10.1002/dvdy.10080
- Issue online: 25 APR 2002
- Version of Record online: 3 APR 2002
- Manuscript Accepted: 8 JAN 2002
- Manuscript Received: 11 SEP 2001
- Heart and Stroke Foundation of Ontario. Grant Number: 4515
- Canadian Genetic Diseases Network
- transgenic mice;
- muscular dystrophy;
- gene expression
Duchenne muscular dystrophy is a muscle wasting disease that results from a dystrophin deficiency in skeletal and cardiac muscle. Studies concerning the regulatory elements that govern dystrophin gene expression in skeletal and/or cardiac muscle in both mouse and human have identified a promoter and an enhancer located in intron 1. In transgenic mice, the muscle promoter alone targets the expression of a lacZ reporter gene only to the right ventricle of the heart, suggesting the need for other regulatory elements to target skeletal muscle and the rest of the heart. Here we report that the mouse dystrophin enhancer from intron 1 can target the expression of a lacZ reporter gene in skeletal muscle as well as in other heart compartments of transgenic mice. Our results also suggest that sequences surrounding the mouse dystrophin enhancer may affect its function throughout mouse development. © 2002 Wiley-Liss, Inc.