Role of VEGF family members and receptors in coronary vessel formation
Article first published online: 3 OCT 2002
Copyright © 2002 Wiley-Liss, Inc.
Volume 225, Issue 3, pages 233–240, November 2002
How to Cite
Tomanek, R. J., Holifield, J. S., Reiter, R. S., Sandra, A. and Lin, J. J.-C. (2002), Role of VEGF family members and receptors in coronary vessel formation. Dev. Dyn., 225: 233–240. doi: 10.1002/dvdy.10158
- Issue published online: 31 OCT 2002
- Article first published online: 3 OCT 2002
- Manuscript Accepted: 23 JUL 2002
- Manuscript Received: 16 APR 2002
- NIH. Grant Number: HL62178
- in situ hybridization
The specific roles of vascular endothelial growth factor (VEGF) family members and their receptors (VEGFRs) in coronary vessel formation were studied. By using the quail heart explant model, we found that neutralizing antibodies to VEGF-B or VEGF-C inhibited tube formation on the collagen gel more than anti–VEGF-A. Soluble VEGFR-1, a receptor for VEGF-A and -B, inhibited tube formation by 87%, a finding consistent with that of VEGF-B inhibition. In contrast, addition of soluble VEGFR-2, a receptor for VEGF family members A, C, D, and E, inhibited tube formation by only 43%. Acidic FGF-induced tube formation dependency on VEGF was demonstrated by the attenuating effect of a soluble VEGFR-1 and -2 chimera. The localization of VEGF R-2 and R-3 was demonstrated by in situ hybridization of serial sections, which documented marked accumulations of transcripts for both receptors at the base of the truncus arteriosus coinciding with the temporal and spatial formation of the coronary arteries by means of ingrowth of capillary plexuses. This finding suggests that both VEGFR-2 and R-3 may play a role in the formation of the coronary artery roots. In summary, these experiments document a role for multiple members of the VEGF family and their receptors in formation of the coronary vascular bed. © 2002 Wiley-Liss, Inc.