Drs. Lin and Geng contributed equally to this work.
T-box binding site mediates the dorsal activation of myf-5 in Xenopus gastrula embryos
Article first published online: 5 DEC 2002
Copyright © 2002 Wiley-Liss, Inc.
Volume 226, Issue 1, pages 51–58, January 2003
How to Cite
Lin, G. F., Geng, X., Chen, Y., Qu, B., Wang, F., Hu, R. and Ding, X. (2003), T-box binding site mediates the dorsal activation of myf-5 in Xenopus gastrula embryos. Dev. Dyn., 226: 51–58. doi: 10.1002/dvdy.10215
- Issue published online: 24 DEC 2002
- Article first published online: 5 DEC 2002
- Manuscript Accepted: 30 SEP 2002
- Manuscript Received: 1 AUG 2002
- National Natural Science Foundation of China. Grant Number: 30170111
- Bureau of Life Sciences and Biotechnology, Chinese Academy of Sciences. Grant Number: KSCX2-2-02
- National Key Project for Basic Research of China. Grant Number: 2001CB50991
- T-box binding site;
- restriction enzyme mediated integration transgenesis
Myf-5, a member of the muscle regulatory factor family of transcription factors, plays an important role in the determination, development, and differentiation of the skeletal muscle. Factors that regulate the expression of myf-5 itself are not well understood. We show here that a T-box binding site in the Xenopus myf-5 promoter mediated the activation of myf-5 expression through specific interaction with nuclear proteins of gastrula embryos. The T-box binding site could be bound by and respond to T-box proteins. T-box genes could induce Xmyf-5. The results suggest that T-box proteins are involved in the specification of myogenic mesoderm and muscle development. © 2002 Wiley-Liss, Inc.