Bone formation during forelimb regeneration: A microtomography (microCT) analysis
Article first published online: 9 JAN 2003
Copyright © 2003 Wiley-Liss, Inc.
Special Issue: Regeneration Special Issue
Volume 226, Issue 2, pages 410–417, February 2003
How to Cite
Stock, S. R., Blackburn, D., Gradassi, M. and Simon, H.-G. (2003), Bone formation during forelimb regeneration: A microtomography (microCT) analysis. Dev. Dyn., 226: 410–417. doi: 10.1002/dvdy.10241
- Issue published online: 28 JAN 2003
- Article first published online: 9 JAN 2003
- Manuscript Accepted: 1 NOV 2002
- Manuscript Received: 28 JUN 2002
- limb regeneration;
- bone formation;
In our study of bone regeneration in the forelimbs of mature newts (Notophthalmus viridescens), we used noninvasive X-ray microtomography (microCT) to image regenerating limbs from 37 to 85 days and matching (contralateral) controls. We compared the patterns of regenerated and existing (nonregenerated) bone, investigating in particular the onset of mineralization of specific bones, the level of mineral present, and the lengths of the different bones. Overall, we find that the missing limb skeletal elements are restored in a proximal-to-distal direction, which reiterates the developmental patterning program. However, in contrast to this proximal–distal sequence, the portion of the humerus distal to the amputation site fails to ossify in synchrony with the regenerating radius and ulna. This finding suggests that the replacement of cartilage with mineralized bone close to the amputation site is delayed with respect to other regenerating skeletal elements. Developmental Dynamics 226:410–417, 2003. © 2003 Wiley-Liss, Inc.