Expression of the Wdr9 gene and protein products during mouse development

Authors

  • He Huang,

    1. McGill Cancer Centre, McGill University, Montréal, Québec, Canada
    2. Department of Medicine (Division of Experimental Medicine), McGill University, Montréal, Québec, Canada
    Search for more papers by this author
  • Isabel Rambaldi,

    1. McGill Cancer Centre, McGill University, Montréal, Québec, Canada
    Search for more papers by this author
  • Eugene Daniels,

    1. Department of Anatomy and Cell Biology, McGill University, Montréal, Québec, Canada
    Search for more papers by this author
  • Mark Featherstone

    Corresponding author
    1. McGill Cancer Centre, McGill University, Montréal, Québec, Canada
    2. Department of Medicine (Division of Experimental Medicine), McGill University, Montréal, Québec, Canada
    3. Department of Anatomy and Cell Biology, McGill University, Montréal, Québec, Canada
    4. Departments of Oncology, McGill University, Montréal, Québec, Canada
    • McGill Cancer Centre, McGill University, 3655 Promenade Sir William Osler, Montréal, QC Canada H3G 1Y6
    Search for more papers by this author

Abstract

Human WDR9 has been mapped to chromosome 21, within one of the Down syndrome (DS) critical regions. Here, we study the expression pattern of the murine Wdr9 gene and its protein product. We show that Wdr9 is broadly expressed in the mouse embryo by means of in situ hybridization and immunohistochemistry. Wdr9 expression levels are dynamic during embryonic development as revealed by Northern blot analysis. We further show that WDR9 is a nuclear protein associated with BRG1, a SWI/SNF complex component. We also demonstrate that a polyglutamine-containing region of the protein functions as a transcriptional activation domain. We propose that WDR9 is a transcriptional regulator involved in chromatin remodeling through the action of two bromodomains and contacts to the SWI/SNF complex. These results may provide a molecular basis for the association of WDR9 with DS. Developmental Dynamics 227:608–614, 2003. © 2003 Wiley-Liss, Inc.

Ancillary